Cellectricon Ab

Piramal Enterprises Limited

Oblique Therapeutics Ab

Fluicell Ab

Collectricon Ab

Nanoxis Consulting Ab

Owe Orwar

Daniel T Chiu

Mattias Karlsson

Kent Jardemark

Sreesha Srinivasa

Peter Eriksson

Aldo Jesorka

Johan Pihl

Alar Ainla

Prabha Mishra

Jessica Olofsson

Carolina Trkulja

Jon Sinclair

Cecilia Farre

Eskil Sahlin

Anders Lundqvist

Richard Neil Zare

Benjamin Pelcman

Kjell Andersson

William Stafford

Frida Ryttsén

Kerstin Nolkrantz

Gavin David Michael Jeffries

Shijun Xu

Max Davidson

Anders Karlsson

Anette Stromberg

Joakim Wigström

Mikael Levin

Jessica Hägglund

Gavin Jeffries

Alexander Moscho

Kerstein Nolkrantz

Vladimir Kirejev

Gavin DM Jeffries

Roger Karlsson

Tatiana Lobovkina

The present invention relates to a pharmaceutical combination comprising a CDK inhibitor and at least one antioxidant enzyme inhibitor for use in the treatment of cancer. The present invention also relates to a method for the treatment of cancer comprising administering to a subject in need thereof, a therapeutically effective amount of a CDK inhibitor and a therapeutically effective amount of at least one antioxidant enzyme inhibitor. The pharmaceutical combination of the present invention exhibits synergistic effect when used in the treatment of cancer.

Pharmaceutical combination for the treatment of cancer

One aspect of the invention provides a system for dispensing and printing cells and particles. The system includes: (a) a flow confinement device; (b) a controller configured to generate a confined liquid volume outside the flow confinement device, wherein the confined liquid volume or materials contained in the confined liquid volume can be released to the environment by confined, modulated, and non-confined flow modes in arbitrary sequence and for arbitrary periods of time by said controller; (c) one or more liquids containing cells or cell constituents supplied into the confined liquid volume through the flow confinement device; (d) a substrate; and (e) a system configured to position the flow confinement device in 3D space and, therefore, the confined liquid volume to be positioned relative to the substrate, such that the confined liquid volume and contents, can interact with the substrate.

Cell printing utilizing recirculating fluid flows

The present invention relates to certain new methods to select epitopes for antibodies. The present invention also provides a method of generating an antibody (e.g. a functional antibody) to a protein, said method comprising (i) identifying an antigenic epitope in said protein by exposing the protein to limited or restricted proteolysis by contacting the protein with at least one protease to form at least one digested, deconstructed or truncated version of the protein and at least one surface-exposed peptide that is cleaved off from the protein by the action of said protease and generating an antigenic epitope based on said surface-exposed peptide; and (ii) raising an antibody against the antigenic epitope. The present invention also provides antigenic epitopes and antibodies against such epitopes.

Methods for epitope selection

The present invention relates to methods of identifying an epitope on a protein that can be bound by an antibody. Methods of the invention typically involve a step of limited or restricted proteolysis of a protein using a single first protease or a combination of first proteases, and a further proteolysis step using a single second protease or combination of second proteases. The invention also relates to identified epitopes and to antibodies which bind to epitopes that have been identified by methods of the invention.

Multi-protease method

There is provided compounds of formula I (I) or pharmaceutically-acceptable salts thereof, wherein L, R, R, R, Rand n have meanings provided in the description, which compounds are useful in the treatment of cancers. 

Sulfinylpyridines and their use in the treatment of cancer

One embodiment of the invention provides a system adapted and configured to generate a localized flow circulation zone. The system includes: a free-standing microfluidic pipette comprising three or more channels with exits separated from each other by an outer surface of the pipette; and a controller programmed to control fluid flows through each of the three or more channels to generate a localized recirculating fluid flow path outside the pipette. Liquid leaving the microfluidic pipette through at least one outlet channel exit is withdrawn through at least two inlet channel exits.

Pipettes, methods of use, and methods of stimulating an object of interest

Aspects of the present invention provide a freestanding microfluidic pipette with integrated wells for solution storage. Further aspects of the invention provide a holding interface to provide connectivity with external control components. One aspect of the invention provides a system for applying a microfluidic device in microscopy. The system includes: a microfluidic device having an elongated shape and defining one or more wells for solution storage and processing; and an interface adapted and configured to hold the microfluidic device in a freestanding manner and facilitate simultaneous connection of the one or more wells with a flow controller. Another aspect of the invention provides a method for utilizing a microfluidic device. The method includes: providing a device as described herein; positioning the device adjacent to a microscope; and actuating the interface to operate the microfluidic device.

Microfluidic device with holding interface, and methods of use

The present invention features a freestanding microfluidic pipette with both solution exchange capability and fluid re-circulation, enabling highly localized and contamination- free fluid delivery within a confined volume in the vicinity of the pipette exit. Preferably, the device features direct positioning, so the pipette can be directed at a point or object of interest, such as a biological or artificial cell, a defined surface area or a sensor element within an open volume, using micro- or nanopositioning techniques. One aspect of the invention provides a free-standing pipette. The free-standing pipette includes a microfluidic device comprising one or more channels with exits leading into an open-volume and a positioning device programmed to hold the microfluidic device at an angle from a horizontal axis.

Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.

Method for selective electrofusion of at least two fusion partners having cell-like membranes

Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.

Method for combined parallel agent delivery and electroporation for cell structures an use thereof

Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.

Method for combined sequential agent delivery and electroporation for cell structures and use thereof

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Hovås, Sweden

Owe Orwar