Mgi Tech Co., Ltd.

Bgi Shenzhen & Bgi Shenzhen Co., Limited

Bgi Shenzhen

Ou Wang

Wenwei Zhang

Xiaofang Cheng

Hui Fu Jiang

Brock A Peters

Weimao Wang

Liangying Zou

Cankun Chang

Fei Fan

Luman Cui

A method for obtaining a single-cell mRNA sequence, including: (1) capturing mRNA of a cell by using a cell tag carrier, and performing reverse transcription to obtain cDNA having a cell tag; (2) obtaining multiple cDNA fragments having molecular tags by using a transposase complex and a molecular tag carrier; (3) performing high-throughput sequencing; (4) performing sequence assembly according to the molecular tags to obtain the sequence of each mRNA; and () obtaining the sequence of all mRNAs of each single cell according to the cell tags.

Method for obtaining single-cell mRNA sequence

Provided is a method of constructing a sequencing library. The method includes 1) providing a single-stranded DNA fragment from a biological sample; 2) subjecting the single-stranded DNA fragment to whole genomic amplification to obtain a whole genome amplification product; 3) fragmenting the whole genome amplification product using a transposase embedded with two adaptors to obtain a fragmented product with two adaptors respectively at two ends; and 4) amplifying the fragmented product with two adaptors respectively at two ends using a tag sequence and a pair of primers to obtain said sequencing library.

Method of constructing sequencing library

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Shenzhen, China

Ou Wang