Eli Lilly and Company

Dowelanco

Otis W Godfrey

Raymond C Yao

Karl H Michel

LaVerne D Boeck

Tom E Eaton

Walter M Nakatsukasa

Hang Chio

Robert L Hamill

David F Mahoney

Richard M Gale

Lawrence E Doolin

Paul Luther Skatrud

Rosanne Bonjouklian

Ralph E Kastner

Thomas D Ingolia

Milton J Zmijewski

Stephen W Queener

Mary L Huber

Tim A Smitka

Ronald D Johnson

Suellen M Samson

James R Ayres

Walter A Raas

Lynda M Ford

Fermentation product A83543, comprising major components A83543A and A83543D and minor components A83543B, A83543C, A83543E, A83543F, A83543G, A83543H and A83543J, is produced by a newly described species, Saccharopolyspora spinosa. The A83543 components and their acid-addition salts (A83543 compounds) are useful as insecticides, particularly against Lepidoptera and Diptera species. Insecticidal, miticidal or ectoparasiticidal combinations, compositions and methods are provided.

Insecticide and miticide compositions containing A83543 compounds

Fermentation product A83543, comprising major components A83543A and A83543D and minor components A83543B, A83543C, A83543E, A983543F, A83543G, A83543H and A83543J, is produced by a newly described species, Saccharopolyspora spinosa. The A83543 components and their acid-addition salts (A83543 compounds) are useful as insecticides, particularly against Lepidoptera and Diptera species. Insecticidal, miticidal or ecto-parasiticidal combinations, compositions and methods are provided.

Insecticide and miticide A83543 compounds and their method of production

Process for producing A83543 compounds

A new microorganism, Streptomyces avermitilis subspecies niger NRRL 21005, and processes using the microorganism for producing known avermectins are provided.

Strain of streptomyces for producing avermectins and processes therewith

Newly-discovered antibiotic A10255 factors B, C, E, and F are produced by submerged aerobic fermentation of previously-unknown Streptomyces gardneri strain NRRL 15922, or an A10255-producing variant, mutant or recombinant thereof. The antibiotics are active against a wide variety of pathogenic bacteria, and also enhance feed-utilization efficiency in chickens, weanling pigs and cattle.

Process for producing the A10255 complex and corresponding microorganism

New glycopeptide antibiotic A80407, comprising A80407A and A80407B, is produced by Kibdelosporangium philippinensis strains NRRL 18198, and NRRL 18199 or A80407-producing mutants of these strains. The A80407 antibiotics have excellent activity against Gram-positive bacteria, increase feed-utilization efficiency in monogastric and ruminant animals and improve weight gains in animals.

A80407 antibiotics

The present invention comprises novel DNA compounds that encode isopenicillin N synthetase and also comprises related methods, transformants, and polypeptides. The novel isopenicillin N synthetase-encoding DNA, together with its associated transcriptional and translational activating sequence, was isolated from Cephalosporium acremonium and cloned into an E. coli cloning vector. The isopenicillin N synthetase-encoding DNA has been used to construct novel E. coli expression vectors that drive expression of a stable, active, and novel isopenicillin N synthetase in E. coli. The intact C. acremonium isopenicillin N synthetase-encoding DNA and associated transcriptional and translational activating sequence have also been used to construct C. acremonium expression vectors that drive expression of the isopenicillin N synthetase in C. acremonium. The C. acremonium transcriptional and translational activating sequence has further been fused to a hygromycin phosphotransferase-encoding DNA segment and placed onto C. acremonium expression vectors. Useful derivatives of the novel compounds and vectors are also described.

Recombinant DNA expression vectors and DNA compounds which encode

Improved fermentation process for producing the gram-positive A-21978C antibiotics which comprises cultivating a new strain of Streptomyces roseosporus, NRRL 15998, and the biologically purified culture of this microorganism are provided.

Process for producing the A-21978C antibiotics

A device for homogenizing a liquid containing a mixture of single cells and cell clusters is provided. The device includes a shroud for channeling the liquid and suspended particles. The shroud defines an inner bore that is formed to include an axial inlet opening and at least one spaced apart outlet opening. A homogenizing bit is provided that is disposed within the inner bore for dispersing the suspended particles in the liquid to form a homogenized substance. The bit includes a shear blade fixed to the bit at a location near the axial inlet opening, with the shear blade configured to cooperate with adjacent shear ports to shear a portion of the suspended particles. The bit also includes a spiral portion that is configured to draw the liquid and suspended particles axially into and through the inner bore from the inlet opening.

Dispersion tool

Antibiotic A-38533 complex, comprising microbiologically active, related factors A.sub.1, A.sub.2, B and C, is produced by submerged, aerobic fermentation of a new Streptomyces sp., NRRL 11298. The A-38533 antibiotics are closely related antibiotics. The individual A-38533 factors are separated by chromatography. The A-38533 factors are antibacterial agents which have a unique activity against Pseudomonas species.

A-38533 Antibiotics and process for production thereof

Growing community of inventors

Greenwood, IN, United States of America

Otis W Godfrey