The General Hospital Corporation

Osman Berk Usta

Mehmet Toner

Martin L Yarmush

Mustafa Korkut Uygun

Maria-Louisa Izamis

Robert Marius Bieganski

Basak Elif Uygun

Timothy Antonie Berendsen

Sinem Perk

Haishui Huang

This disclosure relates to methods of super-cooling of aqueous samples with or without biological samples. The methods involve, e.g., providing a container comprising the aqueous sample; applying an immiscible liquid phase of sufficient thickness to separate the aqueous sample from air; and cooling the aqueous sample to a temperature that is below 0° C.

Methods of supercooling aqueous samples

The present invention generally relates to methods and compositions to determine viability of an organ for transplantation and other medical purposes. One aspect of the invention relates to a method for assessing the viability of an organ by measuring the energy parameters to determine the energy level of the organ by determining the stored cellular energy (e.g., ATP levels), and/or energy consumption over a particular time period of viability. The energy parameters can be compared to reference energy parameters as a highly accurate and reliable prediction of viable cell yield, and organ viability. Another aspect of the invention relates methods to preserve or extend the time period of viability of an organ any combination of (i) preservation perfusion of the organ to prevent ischemic damage, (ii) chemical metabolic suppression of the organ e.g., using metabolic suppressants, (iii) metabolic suppression by physical or environmental conditions, e.g., sub-zero non-freezing storage.

Methods and compositions for preserving tissues and organs

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Watertown, MA, United States of America

Osman Berk Usta