Applied Nanosystems B.v.

Lanthiopep B.v.

Stichting Katholieke Universiteit / Radboud University Nijmegen Medical Centre

Stichting Katholieke Universiteit

Oscar Paul Kuipers

Cornelis Johannes Leenhouts

Jan Kok

Girbe Buist

Ranjan Ramasamy

Anton Steen

Arnold Jacob Driessen

Gert Nikolaas Moll

Rick Rink

Peter Wilhelmus Maria Hermans

Anneke Kuipers

Hester Jeanette Bootsma

Pieter Jan Burghout

Arnold Jacob Mathieu Driessen

Johanna Jacoba Elisabeth Bijlsma

Tomas Gerrit Kloosterman

Johanna Jacoba Bijlsma

Peter Wilhelmus Hermans

The present invention provides proteins/genes, which are essential for survival, and consequently, for virulence ofin vivo, and thus are ideal vaccine candidates for a vaccine preparation against pneumococcal infection. Further, also antibodies against said protein(s) are included in the invention.

Virulence factors of

The invention relates to the production of heterologous polypeptides in a recombinant host cell. More specifically, it relates to the production and secretion of peptides, such as biologically active peptides, that are modified by one or more lantibiotic-synthesizing enzymes. Provided is a nucleic acid construct encoding a polypeptide comprising 1) a non-lantibiotic export signal that is recognized by a non-lantibiotic export system; 2) a lantibiotic leader peptide that is recognized by at least a lantibiotic dehydratase such as LanB and, C-terminally of said export signal and said leader peptide, 3) a peptide of interest containing one or more serine or threonine residue(s) which can be posttranslationally dehydrated by said dehydratase. Also provided is a method for producing a polypeptide in a host cell, comprising providing a host cell with a nucleic acid construct according to the invention and allowing expression and secretion of the encoded polypeptide by said host cell, wherein said host cell comprises at least a dehydratase capable of modifying said encoded polypeptide and a non-lantibiotic export system capable of secreting said modified polypeptide.

Methods for the production and secretion of modified peptides

Methods for improving binding of a proteinaceous substance to cell-wall material of a Gram-positive bacterium are disclosed. The proteinaceous substance includes an AcmA cell-wall binding domain, homolog or functional derivative thereof. The method includes treating the cell-wall material with a solution capable of removing a cell-wall component such as a protein, lipoteichoic acid or carbohydrate from the cell-wall material and contacting the proteinaceous substance with the cell-wall material.

Immunization with bacterial ghost-based vaccines

Method to provide bacterial ghosts provided with antigens

Bacterial ghosts provided with antigens

Growing community of inventors

Groningen, Netherlands

Oscar Paul Kuipers