Syngenta Mogen B.v.

Mogen International Nv

Oscar Johannes Maria Goddijn

Jan Pen

Josephus Christianus Maria Smeekens

Cornelis Maria Petrus Van Dun

Peter Christiaan Sijmons

Teunis Cornelis Verwoerd

Stephan Andreas Ohl

Frederique Marianne Van Der Lee

Eline Voogd

Hendrik Tigelaar

Klaus-Peter Krause

Joke Johanna Catharina Klap

Ronny Wilhelmus Hermanus Henrika Krutwagen

Method for the inhibition of carbon flow in the glycolytic direction in a cell by increasing the intracellular availability of trehalose-6-phosphate.

Regulating metabolism by modifying the level of trehalose-6-phosphate

The invention provides a process for producing trehalose in plant cells capable of producing trehalase by growing plant cells having the genetic information required for the production of trehalose and trehalase, or cultivating a plant or a part thereof comprising such plant cells, characterized in that said plant cells are grown, or said plant or a part thereof, is cultivated in the presence of a trehalase inhibitor.

Enhanced accumulation of trehalose in plants

The invention involves decreasing the intracellular availability of trehalose-6-phosphate by plant cell transformation with a gene encoding trehalose-6-phosphate phosphatase from . Phenotypic effects of plant transformation with this gene include stimulation of glycolysis, cell or tissue growth, and metabolism; and inhibition of photosynthesis and bolting.

The present invention is directed to the production of male sterile plants by providing them with a recombinant DNA capable of specific expression in the male reproductive system of a plant of the enzyme trehalose phosphate (TPP). Resotration of the fertility can be established either by providing said male sterile plants with a recombinant DNA capable of expression of trehalose phosphate synthase (TPS) under control of an inducible promoter or with a recombinant DNA capable of expression of a suppressor protein which suppresses expression of TPP under control of an inducible promoter. This inducible restoration possibilities enable the maintenance of a homozygous male sterile line. Restoration can also be done by spraying the male sterile plants with gibberellic acid. For production of hybrids or hybrid seed a site-specific recombination system is provided, by inserting two site=specific recombination sites flanking the recombinant DNA coding for TPP and crossing the male sterile lines with lines expressing the corresponding recombinase. By crossing the recombinase will excise the gene coding for TPP and fertile hybrids are produced.

Nuclear male sterile plants, method of producing same and methods to restore fertility

This invention describes a method to prevent post-harvest sprouting in potato by transforming the potato plant with a gene coding for trehalose phosphate synthase.

Pre- and postharvest inhibition of remobilisation of storage compounds

An isolated DNA fragment that promotes root knot or cyst nematode indcuible transcription of a coding sequence downstream of and operably linked to the fragment in at least an,plant and a chimeric DNA sequence including the DNA fragment. Also, methods for the use of the DNA fragment.

Growing community of inventors

Leiden, Netherlands

Oscar Johannes Maria Goddijn