Sanwa Kagaku Kenkyusho Co., Ltd.

Osamu Takehiro

Masayasu Kurono

Kiichi Sawai

Shizuo Uno

A test strip including: a first site that's a detection site, where a first antibody specifically binding to the substance to be measured is immobilized on a porous carrier; a second site that's a specimen liquid addition site, where a second labeled antibody binding to the substance to be measured and having a recognition site different from that of the first antibody is retained on a porous carrier to be movable; and a third site that is a specimen liquid or developer addition site, where colored microparticles bound with a substance binding to a label substance of the second antibody are retained on a porous carrier to be movable, wherein the second site is provided between the first and the third sites, and the specimen liquid or developer can move, due to capillarity, from the second site to the first and from the third to the first site.

Immunochromatography device

A method of enzymatically measuring hydrogen peroxide by developing a color with use of 3-methyl-2-benzothiazolinoehydrazone as a coupler and in combination with an oxidative color developing reagent with peroxidase, and a reagent therefor is described. Catalase is added to the 3-methyl-2-benzothiazolinonehydrazone reagent as a stabilizer and the reaction is carried out in the presence of ethylenediaminetetraacetic acid or an analogue thereof. The color developing reaction may be carried out in neutral or weak alkali conditions. A reagent is described which comprises a buffer, 3-methyl-2-benzothiazolinohydrazone, and catalase.

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Inabe, Japan

Osamu Takehiro