Fuji Photo Film Company, Limited

Fujifilm Corporation

Fuju Photo Film Co., Ltd.

Osamu Seshimoto

Kenichiro Yazawa

Masaaki Terashima

Takaki Arai

Hiroshi Shinoki

Akira Yamaguchi

Mitsuharu Nirasawa

Toshihisa Ito

Teruaki Koizumi

Shunichi Seto

Yasushi Fujisaki

Fumiaki Shinozaki

Tomoaki Ikeda

Asaji Kondo

Tomizo Namiki

Hideo Sato

Masashi Ogawa

Kentaro Nakamura

Masao Kitajima

Yoshio Saito

Yoshikazu Amano

Yukio Sudo

Yoshihide Iwaki

Shigemitsu Mizutani

Yoshihiko Abe

Masayoshi Kojima

Kikuo Kubotera

Shigeru Tezuka

Hikaru Tsuruta

Hiroko Inomata

Kimiko Ishizuka

Toshihiro Mori

Seiji Horie

Akira Nahara

Takashi Koizumi

Junji Nishigaki

Yoshihiro Seto

Yoshihiko Makino

Kiyoshi Yamada

Kaoru Terashima

Takehiko Sato

Yuzo Mizobuchi

Masaaki Takimoto

Keishiro Kido

Yoshiki Sakaino

Kouki Nakamura

Sigeru Tezuka

Tohru Sueyoshi

Takeshi Igarashi

Kazuya Kawasaki

Keiichi Ishizaki

Takeshi Takayama

Hirotsugu Nomaguchi

Kenji Miura

Hiroyuki Amano

Hitomi Ito

Mituharu Nirasawa

Sadao Yamada

Shohei Oda

The immunoassay element for quantitatively analyzing an antigen by determining the change in enzymatic activity of an enzyme-labelled antigen or antibody caused by an immunological reaction. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the labelling enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. As the non-diffusible substrate, a substrate capable of reacting solely with the labelling enzyme and incapable of reacting the fragmenting enzyme is utilized. When an endo-active glucosidase is used as the labelling enzyme, and an exo-active glucosidase is used the fragmenting enzyme in the reagent layer, the non-diffusible substrate of the substrate layer is preferred to be an endo type selectively reactive substrate, which means a substrate having a reactivity specific to endo-active glucosidase. Highly sensitive assay is realized with high accuracy and high reproducibility and good storage stability.

Immunoassay element

An object of the present invention is to suppress an electric potential drift as described above and improve pH measurement precision in order to obtain practical measurement precision in the dry-type multilayered film-type pH electrode. The present invention provides a complex pH electrode which has at least two ion-selective electrodes comprising a non-conductive support, a pair of electrode layers, an electrolytic layer and an ion-selective membrane where at least one of the ion-selective electrode is a hydrogen ion-selective electrode, wherein the hydrogen ion-selective membrane is saturated with carbon dioxide gas.

PH electrode

The present invention provides a method for detecting fluorescence by using a solid support to which a probe molecule to be detected is fixed, wherein background is reduced by using a quenching agent. By using present invention, detection sensitivity of a DNA chip can be increased and stable data can be obtained.

Method of reducing background in fluoresence detection

An object of the present invention is to provide a means for simply performing a gene analysis without performing complicated operations. The present invention provides a method for the detection of a nucleic acid, comprising the steps of: performing a PCR reaction or a reverse transcription reaction by adding a template nucleic acid to a solid support on which a nucleic acid is fixed; and hybridizing the nucleic acid fixed on said solid support with the nucleic acid synthesized by the PCR reaction or the reverse transcription reaction.

Method for the detection of gene with DNA micro-array

An ion selective monoelectrode complex which is favorably employable to manufacture an ion activity measuring apparatus, has on a common non-electroconductive support sheet, plural ion selective monoelectrodes each of which is composed of an electrode composite consisting of, in order, a silver metal layer, a silver halide layer, an electrolytic material layer, and an ion selective membrane, and an electroconductive terminal which is electrically connected to the silver metal layer and which has an exposed surface, under the condition that the ion selective monoelectrodes are aligned, without electric contact with each other, along an imaginary line bridging the electrode composite and the electroconductive terminal.

Ion selective monoelectrode complex

According to the present invention, there is provided a fluorescent nucleotide represented by the formula: A-B-C, wherein A represents a residue of natural or synthetic nucleotide, oligonucleotide, polynucleotide, or derivative thereof, and binds to B at a base moiety in said residue; B represents a divalent linking group or a single bond; and C represents a monovalent group derived from a fluorescent dye having 0 or 1 sulfonic acid group or phosphoric acid group in a molecule. The present invention provides useful fluorescent nucleotides for labeling nucleic acids, specifically, fluorescent nucleotides of which uptake ratio is high in synthetic reaction of nucleic acids.

Fluorescent nucleotides

This invention provides a continuous blood filtration apparatus capable of filtering a plurality of blood samples continuous by using blood filter units to prepare plasma or serum samples in a short period, which comprises, a conveyor conveying a plurality of blood reservoirs each of which contains a blood sample and in which a suction nozzle of a blood filter unit has been put, a manifold connected to a suction line, couples of a valve and a connector for connecting the manifold to the blood filter unit provided on the end of each branch of the manifold, and a mechanism of moving the blood reservoirs in vertical direction.

Continuous blood filtration apparatus

A device for measuring ionic activity is composed of:

Device for measuring ionic activities

A process for blocking a device for detection of biochemically active molecules is performed by the steps of:

Blocking of device for detecting biochemically active molecules

A composite ion selective electrode comprising a calcium ion selective electrode member and a hydrogen ion selective electrode member which is characterized in that a calcium ion selective membrane of the calcium ion selective electrode member has a thickness of 5 to 30 &mgr;m, and the hydrogen ion selective membrane of the hydrogen ion selective electrode member contains tri-n-dodecyl-amine and trisethylhexyl trimellitate. Each of the calcium ion selective electrode member and the hydrogen ion selective electrode member can be employed singly.

Ion selective electrode

The present invention provides a measurement chip for a surface plasmon resonance biosensor, which comprises a transparent substrate, a metal membrane located on the transparent substrate and an organic silicon membrane immobilized on the metal membrane and in which the organic silicon membrane is immobilized on the metal membrane via a functional group capable of binding with atoms on the surface of a metal. The measurement chip for a biosensor of the present invention can easily be produced. Using the measurement chip of the present invention, a target substance can be measured with good sensitivity, even if only a small amount of physiologically active substance is immobilized.

Measurement chip for surface plasmon resonance biosensor

A DNA chip or its analogue composed of nucleotide derivatives attached to a solid carrier is prepared by method of bringing nucleotide derivatives having a reactive group at each one terminal into contact with a solid carrier having thereon reactive groups in an aqueous phase in the presence of a transferase which is capable of producing a covalent bond by rearrangement of the reactive group of the nucleotide derivative and the reactive group of the solid carrier.

Fixation of nucleotide derivatives to solid carrier

Fluorescent substances

In the manufacture of an ion selective electrode composed of a non-electroconductive support, a pair of electrodes each of which has a silver metal layer and a silver halide layer, an electrolytic material layer, an ion selective membrane, and a non-electroconductive sheet having a pair of openings for receiving a sample solution and a reference solution, respectively, and having thereon a bridge member for electrically connecting the sample solution received in one opening and the reference solution received in another opening, the ion selective membrane is advantageously divided into two separate portions, one of which is placed in a position above one electrode and another of which is placed in a position above another electrode.

Growing community of inventors

Saitama, Japan

Osamu Seshimoto