Kazusa Dna Research Institute Foundation

Kazusa Dna Research Institute

Chugai Seiyaku Kabushiki Kaisha

Daiichi Sankyo Company, Limited

Aisin Cosmos R&d Co., Ltd.

Riken Corporation

Other

Shimadzu Corporation

Nara Institute of Science and Technology

The Jackson Laboratory

Nippon Zenyaku Kogyo Co., Ltd.

Altair Corporation

Animal Allergy Clinical Laboratories Inc.

Nano Fusion Technologies, Inc.

Astellas Pharma Gmbh

Osamu Ohara

Takashi Watanabe

Takahiro Nagase

Michio Oishi

Shin-Ichi Funahashi

Hiroshi Yokota

Nobuo Nomura

Shoji Miyata

Kazuhiro Kondo

Tetsuo Ohashi

Yasushi Shigemori

Daisuke Okajima

Haruhiko Koseki

Mitsuhiro Tada

Hiroki Arakawa

Osamu Kamida

Akiyoshi Uemura

Mitsuhiro Okada

Hisashi Koga

Daisuke Nakajima

Ken Nonaka

Michio Ohishi

Kouichi Nishimura

Jun Yanagibayashi

Takehiko Shibata

Leonard D Shultz

Shin-ichiro Fujii

Toshihiro Tsukui

Kunitake Abe

Kanako Shimizu

Kenichi Masuda

Noboru Yamaji

Tsutomu Mikawa

Yoriko Saito

Fumihiko Ishikawa

Ichiro Yanagisawa

Reiko Ohara

Yoshihiro Yamaguchi

Mamoru Hirafuji

Ichiro Tominaga

Reiko Kikuno

Shigeto Eda

Naoyuki Inagaki

Tatsuya Mori

Atsushi Hijikata

Hiroshi Kitamura

Hidetoshi Ozawa

The purpose of the present invention is to provide a simple measurement tool which can rapidly measure various chemical substances contained in environments or from inside a living body by a simple or inexpensive means/method. The present inventions relate to, for example, a simple measurement tool for test substances comprising:

Simple measurement tool having reaction regions separated by gel boundary regions

The present invention provides use of KLRG1 as a marker specific to memory invariant NKT cells. Using an antibody that specifically recognizes KLRG1, memory invariant NKT cells can be easily detected or isolated.

Memory invariant NKT cell marker

The present invention relates to a particle manipulation method to disperse magnetic particlesin a liquidfilling up a tube container, wherein a circumferential direction moving step to move the magnetic particlesalong the circumferential direction of the containerin the liquidand in a radial direction moving step to move the magnetic particlesas crossing the radial direction of the containerin the liquidare implemented repeatedly. Such manipulations can be achieved by combining rotation of the container and gravity force and magnetic field manipulations.

Particle operation method and particle operation device

Disclosed is a particle manipulation method including steps of moving particles, which exist in a water-based liquid, into a gelled medium that is insoluble or hardly soluble in the water-based liquid in the water-based liquid, and moving the particles, which exist in the gelled medium, to the outside of the gelled medium. Preferably, the gelled medium is a gel that contains chemically crosslinking polymer. Preferably, the gelled medium has a consistency of 340 to 475. In one embodiment, the movement of the particles existing in the water-based liquid to the gelled medium and the movement of the particles existing inside the gelled medium to the water-based liquid are carried out inside a device, the device being loaded with a plurality of water-based liquids and a gelled medium interposed among the water-based liquids.

Particle manipulation method and particle manipulation device

Problem to be Solved: The present invention is intended to provide a polynucleotide encoding the light-chain variable region and the heavy-chain variable region of an anti-dog IgE antibody; and an anti-dog IgE antibody containing these variable regions. Solution: The present invention is DNA encoding a heavy-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 2 or 6 and DNA encoding a light-chain variable region consisting of the amino acid sequence represented by SEQ ID NO: 4 or 8, and an anti-dog IgE monoclonal antibody which binds to dog IgE, containing these variable regions or a functional fragment thereof which binds to dog IgE.

Anti-dog IgE monoclonal antibody, and light-chain variable region and heavy-chain variable region of anti-dog IgE monoclonal antibody

The invention relates to a method of selectively expanding human leukemic cells in a non-adult NOD/SCID/IL2rgmouse by transplanting a substance containing a leukemic stem cell derived from a human acute myelogenous leukemia patient to the mouse. In addition, the invention relates to screening for a medicament capable of eradicating leukemic stem cell (LSC), consideration of treatment methods suitable for individual patients, identification of a differentially expressed gene and the like, using a mouse with expanded human leukemic cells.

Mouse having human leukemic stem cell and leukemic non-stem cell amplified therein, and method for production thereof

A pipette core member is used in a pipette for sampling a sample. This pipette core member includes: a pump body including a reservoir communicated with one suction/discharge port of an electroosmotic flow pump, the electroosmotic flow pump and the reservoir being integrally formed; and a capillary connected to the electroosmotic flow pump and communicated with the other suction/discharge port of the electroosmotic flow pump. The capillary is secured to the pump body.

Pipette core member, pipette, and pipette device

A gene encoding a novel protein that works as a guanine nucleotide exchange factor (GEF) for a Rho family protein being one group of small GTP-binding proteins, namely, a polynucleotide shown by the nucleotide sequence set forth in SEQ ID NO: 1, SEQ ID NO: 3, or SEQ ID NO: 5, or the complementary strand, the equivalents of the polynucleotide, a protein encoded by the polynucleotide, a vector containing the polynucleotide, a transformant containing the vector, an antibody against the protein encoded by the polynucleotide, a method of identifying a compound that inhibits the function of the protein encoded by the polynucleotide and/or the expression of the polynucleotide, a method of determining a disease, a pharmaceutical composition, and a reagent kit are provided.

Antibody to a guanine nucleotide exchange factor

Singar is identified as a novel molecule, whose expression is varied before and after the polarization of a nerve cell, and occurs in the tip of an elongating axon, called a growth cone, which is important for the formation or elongation of an axon. Singar is expressed specifically in the brain and the amount of Singar expression is largely increased in an individual during periods where the formation of axons is increased. It is observed that Singar is highly enriched in the growth cone at the tip of an axon. When the expression of Singar is inhibited in nerve cells in culture, the formation of multiple axons is induced. Thus, the inhibition of Singar can induce axon formation. Therefore, by inhibiting the expression or activity of Singar, it becomes possible to induce or promote the formation or elongation of an axon in a nerve cell.

Formation/elongation of axon by inhibiting the expression or function of Singar and application to nerve regeneration

The present invention is related to a DNA comprising a nucleotide sequence encoding a polypeptide represented by SEQ ID NO:1 or SEQ ID NO:2. The DNA according to the present invention is highly expressed in prostatic adenocarcinoma and ovarian carcinoma, and is a cancer-associated gene, so that it is possible to inhibit cancer by blocking the binding of the present protein to its ligand. Accordingly, the present antibody is used not only in the detection of the present protein, but also as an agent for the treatment or prevention of cancers such as prostatic adenocarcinoma and ovarian carcinoma.

Cancer-associated genes

DNA encoding a cholecystokinin octapeptide sulfated form (CCK-8S) G-protein coupled receptor, a complementary strand thereof, a vector containing the DNA, a transformant containing the vector, a pharmaceutical composition, and a reagent kit are provided. Such DNA and constructs are useful in identifying a compound that inhibits or promotes a function of the protein and/or expression of a DNA encoding the protein. Such DNA and constructs are also useful for identifying an anti-depressant drug or a compound that has an anti-depressant action.

DNA encoding a CCK-8S G-protein coupled receptor, and vectors, transformants, cell kits, and methods employing such DNA

A novel gene apparently encoding a transmembrane glycoprotein has been successfully isolated by constructing a cDNA library of 4 kb or above in size from mRNA expressed in human adult brain and analyzing the structures of the cDNAs contained within said library by the shotgun method. The novel gene shows brain-specific expression and the protein encoded by said gene has a typical PDZ binding motif.

Antibodies to brain specific membrane protein

The present invention is directed to a novel plexin family like DNA, a gene containing the DNA, novel polypeptide encoded by the DNA, a recombinant protein containing the polypeptide or heterologous polypeptide sequences, antibodies which bind to the polypeptides, methods for producing the polypeptides of the present invention. Conditions that can be diagnosed, screened, prevented, or treated by the compositions herein include prophylactic, angiogenesis and conservation of functions in various organs, angiogenesis at the time of tumor and regeneration of various organs, proliferation, differentiation and preservation of function of the cells in various organs, aging, and dysfunction of the present invention.

DNA encoding plexin polypeptides and kits thereof

The present invention provides: a novel DNA, a carcinoma-associated gene comprising the DNA, a recombinant protein encoded by the DNA, an antibody binding to the protein, an anti-carcinoma agent comprising the antibody, a low-molecular-weight compound binding to the protein, and a screening system. An example of such a novel DNA is a DNA comprising a nucleotide sequence encoding the following polypeptide (a) or (b):

Cancer-associated gene

An object of the present invention is to provide a nucleic acid amplification method for amplifying a desired nucleic acid while suppressing amplification of byproducts in a PCR reaction, a reagent kit used for nucleic acid amplification, a method of detecting single nucleotide polymorphism to detect single nucleotide polymorphism by utilizing that amplification of byproducts is suppressed in a PCR reaction, and a reagent kit used for detecting single nucleotide polymorphism. The method of amplifying nucleic acids by PCR is characterized by admixing in a reaction solution, a homologous recombinant protein which contains at least one of a RecA protein derived from, and a modified RecA protein obtained by modification of the RecA protein and having a function similar to that of the RecA protein, and carrying out PCR.

Method of amplifying nucleic acids, reagent kit for amplifying nucleic acids, method of detecting single nucleotide polymorphism, and reagent kit for detecting single nucleotide polymorphism

Growing community of inventors

Chiba, Japan

Osamu Ohara