Ajinomoto Co., Ltd.

Osamu Kurahashi

Tsuyoshi Nakamatsu

Hisao Ito

Eiichiro Kimura

Yoshio Kawahara

Hisashi Kawasaki

Kazuhiko Matsui

Yukiko Mori

Kenichi Hashiguchi

Yoshihiro Usuda

Shinichi Sugimoto

Yoshimi Kikuchi

Yoko Asakura

Megumi Shimaoka

Yumi Matsuzaki

Hiroshi Matsui

Takayasu Tsuchida

Yasuhiro Takenaka

Hitoshi Enei

Mikiko Suga

Hiroki Kawashima

Yoko Kuwabara

Shigeru Nakamori

Jun Nakamura

Yasuhiro Mihara

Seiko Hirano

Gen Nonaka

Tsuyoshi Osumi

Naoki Akiyoshi

Kanae Nakamura

Fusao Tomita

Nobuharu Tsujimoto

Tsuyoshi Ohsumi

Masako Izui

Atsushi Yokota

Sohei Kanno

Shinya Goto

Masako Ishigooka

Nobuharu Tujimoto

Yoshiko Kawahara

Kenzo Yokozeki

Hiroyuki Kojima

Takashi Tanaka

Kiyoshi Miwa

Hiroshi Izui

Konosuke Sano

Yasuhisa Asano

Masakazu Sugimoto

Kazuo Nakanishi

Yuta Nakai

Eiji Ono

Haruo Momose

Kohki Ishikawa

Ei-ichiro Suzuki

Nobuhisa Shimba

Yukiko Ono

Keiko Gondoh

Tohru Kouda

Hiroko Kishino

Chizu Abe

Kayo Moriguchi

Yuri Miyata

Rie Kozutsumi

Masahiro Kamada

In a method for producing a target substance utilizing a microorganism comprising culturing the microorganism in a medium to produce and cause accumulation of the target substance in the medium and collecting the target substance, there is used, as the microorganism, a mutant strain or a genetic recombinant strain constructed from a parent strain of the microorganism having a respiratory chain pathway of high energy efficiency and a respiratory chain pathway of low energy efficiency as respiratory chain pathways, and having either one or both of the following characteristics:

Method for producing substance utilizing microorganism

The present invention provides a method for producing L-methionine by culturing a microorganism in a medium to produce and accumulate L-methionine in the medium, and collecting the L-methionine from the medium, where the microorganism is deficient in a repressor of L-methionine biosynthesis system and has L-methionine productivity.

Method for producing L-methionine by fermentation

A microorganism which has a gene encoding an enzyme in which feedback inhibition is desensitized by substitution of one or two amino acids in PRPP amidotransferase encoded by purF of, a gene encoding a protein which is an inactivated repressor of purine nucleotide biosynthesis encoded by purR, a gene encoding an enzyme which is inactivated purine nucleoside phosphorylase encoded by deoD, a gene encoding an enzyme which is inactivated succinyl-AMP synthase encoded by purA, a gene encoding an enzyme which is inactivated 6-phosphogluconate dehydrase encoded by edd, a gene encoding an enzyme which is inactivated phosphoglucose isomerase encoded by pgi and like is bred and a purine nucleoside is produced by culturing the microorganism.

Method for producing purine nucleoside by fermentation

L-Methionine is produced by culturing a microorganism which is deficient in repressor of L-methionine biosynthesis system and/or enhanced intracellular homoserine transsuccinylase activity is cultured in a medium so that L-methionine should be produced and accumulated in the medium, and collecting the L-methionine from the medium. The microorganism preferably further exhibits reduced intracellular S-adenosylmethionine synthetase activity, L-threonine auxotrophy, enhanced intracellular cystathionine &#x3b3;-synthase activity and enhanced intracellular aspartokinase-homoserine dehydrogenase II activity. The present invention enables breeding of L-methionine-producing bacteria, and L-methionine production by fermentation.

A method of producing coryneform bacteria having an improved amino acid or nucleic acid-productivity comprises the steps of introducing a mutation in a promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on a chromosome of a coryneform bacterium so that it is close to a consensus sequence or introducing a change in the promoter sequence of amino acid- or nucleic acid-biosynthesizing genes on the chromosome of a coryneform bacterium by gene recombination so that it is close to a consensus sequence, obtaining mutants of the coryneform amino acid- or nucleic acid-producing microorganism, culturing the mutants and select a mutant capable of producing the intended amino acid or nucleic acid in a large amount. This method allows one of skill in the art to construct a mutant capable of enriching or controlling the expression of an intended gene without using a plasmid and to promote production of amino acids in a high yield, by the recombination or mutation.

Method of constructing amino acid producing bacterial strains, and method of preparing amino acids by fermentation with the constructed amino acid producing bacterial strains

L-Arginine is produced by culturing a coryneform bacterium in which an arginine repressor involved in L-arginine biosynthesis is deleted by disrupting a gene coding for the repressor, and which has L-arginine producing ability in a medium to produce and accumulate L-arginine in the medium, and collecting the L-arginine from the medium.

Arginine repressor deficient strain of coryneform bacterium and method for producing L-arginine

A DNA fragment which encodes a polypeptide defined in the following (a) or (b), and a polypeptide defined in the following (c) or (d):

Carbamoyl-phosphate synthetase gene of coryneform bacteria and method for producing L-arginine

L-Glutamine is produced by culturing a coryneform bacterium which has L-glutamine producing ability and has been modified so that its intracellular glutamine synthetase activity should be enhanced, preferably which has been further modified so that its intracellular glutamate dehydrogenase activity should be enhanced, in a medium to produce and accumulate L-glutamine in the medium and collecting the L-glutamine.

Method for producing L-glutamine by fermentation and L-glutamine producing bacterium

A plasmid isolatable from, which comprises a gene coding for a Rep protein having the amino acid sequence shown in SEQ ID NO: 8 or an amino acid sequence having homology of 90% or more to the amino acid sequence shown in SEQ ID NO: 8, and has a size of about 4.4 kb or about 6 kb, or a derivative thereof.

Plasmid autonomously replicable in coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived from, preferably an enzyme that functions at a higher temperature compared with that of. PCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

Genes for heat resistant enzymes of amino acid biosynthetic pathway derived from thermophilic coryneform bacteria

A plurality of primer sets are designed based on a region where conservation at the amino acid level is observed among various microorganisms for known gene sequences corresponding to a gene coding for an enzyme of the L-amino acid biosynthetic pathway derived frompreferably an enzyme that functions at a higher temperature compared with that ofPCR is performed by using the primers and chromosomal DNA ofas a template. The primers with which an amplification fragment has been obtained are used as primers for screening to select a clone containing a target DNA fragment from a plasmid library of chromosomal DNA of

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Kawasaki, Japan

Osamu Kurahashi