The General Hospital Corporation

The Charles Stark Draper Laboratory, Inc.

Osamu Ishii

Jeffrey T Borenstein

Joseph P Vacanti

Eli J Weinberg

Mohammad Reza Kaazempur-Mofrad

Kevin R King

Jennifer Ogilvie

Chiao-Chun Wang

Alexander Sevy

Young-Moon Michael Shin

Tomoyuki Maemura

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick, structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Fabrication of vascularized tissue using microfabricated two-dimensional molds

Methods and materials for making complex, living, vascularized tissues for organ and tissue replacement, especially complex and/or thick structures, such as liver tissue is provided. Tissue lamina is made in a system comprising an apparatus having (a) a first mold or polymer scaffold, a semi-permeable membrane, and a second mold or polymer scaffold, wherein the semi-permeable membrane is disposed between the first and second molds or polymer scaffolds, wherein the first and second molds or polymer scaffolds have means defining microchannels positioned toward the semi-permeable membrane, wherein the first and second molds or polymer scaffolds are fastened together; and (b) animal cells. Methods for producing complex, three-dimensional tissues or organs from tissue lamina are also provided.

Fabrication of tissue lamina using microfabricated two-dimensional molds

Growing community of inventors

Arlington, MA, United States of America

Osamu Ishii