Kikkoman Corporation

Noda Institute for Scientific Research

Noda Institutute for Scientific Research

Osamu Hatamoto

Eiichi Nakano

Masayuki Machida

Tsutomu Masuda

Kiyoshi Mizusawa

Motoaki Sano

Kenichiro Matsushima

Genryou Umitsuki

Teruo Watarai

Tomomi Uchikawa

Chitoshi Oka

Genryou Umitsüki

Hiroshi Maeda

Hitoshi Tainaka

Akimitsu Tanaka

Touru Ito

Tomomi Ichikawa

To improve the activity of a Koji mold protease in a solid or liquid culture medium in the production of foods (e.g., a seasoning), pharmaceuticals (e.g., a digestive agent), protease for use in a detergent and the like. Disclosed are a recombinant vector having capability of increasing the secretion of the Koji mold protease, a Koji mold which is transformed with the vector and has an increased expression of a gene for a protease or an increase secretion of the same, a method for the production of a protease by using the transformed Koji mold, and the like.

Recombinant vector capable of increasing secretion of Koji mold protease

Coating material

The present invention relates to a DNA fragment of 3,563 base pairs containing a gene coding for tannase and derived from a microorganism belonging to the genus Aspergillus, with the following restriction enzyme map: ##STR1## B: Bam HI, H: Hind III, K: Kpn I, S: Sal I, X: Xba I; a DNA fragment containing a tannase gene coding for the amino acid sequence of (SEQ ID NO:4); a recombinant plasmid comprising the DNA fragment containing the tannase gene inserted into a plasmid vector; a process for producing tannase, comprising culturing a microorganism belonging to the genus Aspergillus capable of producing tannase in medium with the recombinant plasmid, and recovering tannase from the culture; and a promoter represented by the nucleotide sequence of (SEQ ID NO:1). Tannase can be efficiently produced according to the present invention.

DNA fragment containing a tannase gene, a recombinant plasmid, a process

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Noda, Japan

Osamu Hatamoto