Seikagaku Kogyo Kabushiki Kaisha

Seikagaku Corporation

Seikagaku Kogyo Kabushiki Kaish

Osami Habuchi

Masakazu Fukuta

Koji Kimata

Takashi Muramatsu

Kenji Kadomatsu

Hiroko Habuchi

Reiji Kannagi

Kenji Uchimura

Hideki Muramatsu

Hirofumi Nakano

Toshihiko Sawada

Sonoko Fujii

Shiori Ohtake

Yukie Hirahara

Shinobu Yamauchi

Masashi Kobayashi

Hirofumi c/o Aichi Univ Of Education Nakano

A galactosamine derivative represented by the following formula (1): wherein R, Rand Reach independently represents SOor H, and at least one of them represents SO; Rrepresents H, acetyl or SO; Rrepresents H, a substituted or unsubstituted alkyl group, a substituted or unsubstituted alkenyl group, a substituted or unsubstituted alkynyl group, a substituted or unsubstituted acyl group, a substituted or unsubstituted aryl group, or a substituted or unsubstituted aralkyl group; X represents O, S, NH or CH; andrepresents an &#x3b1; bond or a &#x3b2; bond, and a sulfotransferase inhibitor comprising the derivative.

Sulfotransferase inhibitors

Polypeptide of N-acetylglucosamine-6-O-sulfotransferase and DNA encoding the same

A galactosaminoglycan 4-sulfotransferase having an activity of transferring a sulfate group from a sulfate group donor to a hydroxyl group at the C-4 position of galactosamine residue of a galactosaminoglycan is presented.

C-4 specific sulfotransferase

A polypeptide of keratan sulfate 6-sulfotransferase having the following physicochemical properties:

Keratan sulfate 6-sulfotransferase and DNA coding for the same

Apolypeptide of N-acetylglucosamine-6-O-sulfotransferase and a DNA encoding the peptide are provided. The polypeptide is (a) or (b) below:

Polypeptide of N-acetylglucosamine-6-O-sulfotransferase and DNA encoding

Disclosed is a method for producing a sulfated lactosamine oligosaccharide, comprising the step of allowing a sulfotransferase to act on a lactosamine oligosaccharide, the sulfotransferase transferring sulfate group to hydroxyl group at C-6 position of galactose residue in the lactosamine oligosaccharide.

Method for producing sulfated lactosamine oligosaccharide

A polypeptide of glycosaminoglycan sulfotransferase originating from human and having the following physical and chemical properties:

Polypeptides of glycosaminoglycan sulfotransferase originating from

The present invention provides a heparan sulfate 6-O-sulfotransferase for selectively introducing sulfate group into hydroxyl group at C-6 of glucosamine of heparin and heparan sulfate, having the following properties: (i) sulfate group is selectively transferred from a sulfate group donor to hydroxyl group at C-6 of N-sulfoglucosamine residue; (ii) sulfate group is transferred to CDSNS-heparan, but sulfate group is not transferred to chondroitin and chondroitin-4-sulfate; (iii) optimum reaction pH is in a range of pH 6-7; (iv) optimum ionic strength is in a range of 0.1-0.3 M (in the case of sodium chloride); and (v) the enzyme activity is inhibited by dithiothreitol and adenosine-3',5'-diphosphate, and activated by protamine.

Heparan sulfate 6-O-sulfotransferase

Chondrotin 6-sulfotransferase (C6ST), for transferring sulfate group to the hydroxyl group at C-6 position of N-acetylgalactosamine residue or galactose residue of glycosaminoglycan, was purified from a culture liquid of chick embryo chondrocytes to determine its partial amino acid sequences. Partial cDNA of C6ST was amplified from poly(A).sub.+ prepared from the chondrocytes, by means of PCR by using oligonucleotide primers prepared on the basis of the determined sequences. Entire length cDNA of C6ST was obtained from a cDNA library by means of hybridization by using an obtained cDNA fragment as a probe.

DNA coding for sulfotransferase

Heparan sulfate 2-O-sulfotransferase having the following properties is obtained from, for example, CHO cells:

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Nagoya, Japan

Osami Habuchi