Qiagen Gmbh

The Public Health Research Institute of the City of New York, Inc.

Osama A Alsmadi

Roger S Lasken

Mark Driscoll

Frank B Dean

A Fawad Faruqi

Seiyu Hosono

Linhua Fang

Michael Egholm

Fred Russell Kramer

Sanjay Tyagi

Michele Wisniewski

Carole Bornarth

Arumugham Raghunathan

Wanmin Song

Fawad A Faruqi

Mark D Driscoll

Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a complex nucleic acid sample such as genomic samples using one, a few, or more primers such that, during replication, the replicated strands are displaced from the nucleic acid molecules in the sample by strand displacement replication of another replicated strand. It was discovered that highly complex nucleic acid samples can be efficiently amplified using only one or a few primers having specific nucleic acid sequences. The one or few primers are complementary to nucleic acid sequences distributed throughout nucleic acid in the sample.

Nucleic acid amplification

Coalescence of cells or other membrane-bound entities is facilitated by anchoring an outwardly projecting first oligonucleotide in one member and an outwardly projecting second oligonucleotide, complementary to the first, in a second member and incubating under hybridizing conditions. Liposomes may be coalesced with cells to deliver hydrophilic agents thereto, such as DNA probes or drugs. Kits containing complementary oligonucleotides containing hydrophobic anchoring moieties may be used.

Oligonucleotide-facilitated coalescence

Disclosed are compositions and a method for amplification of nucleic acid sequences of interest. The disclosed method generally involves replication of a target sequence such that, during replication, the replicated strands are displaced from the target sequence by strand displacement replication of another replicated strand. In one form of the disclosed method, the target sample is not subjected to denaturing conditions. It was discovered that the target nucleic acids, genomic DNA, for example, need not be denatured for efficient multiple displacement amplification. The primers used can be hexamer primers. The primers can also each contain at least one modified nucleotide such that the primers are nuclease resistant. The primers can also each contain at least one modified nucleotide such that the melting temperature of the primer is altered relative to a primer of the same sequence without the modified nucleotide(s). The DNA polymerase can be &#x3c6;29 DNA polymerase.

Amplification of denatured and stabilized nucleic acids

Multiple displacement amplification

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Guilford, CT, United States of America

Osama A Alsmadi