Annikki Gmbh

Novolana Gmbh

Ortwin Ertl

Bernd Mayer

Nicole Staunig

Marta Sut

Kurt Messner

Karin Fackler

Thomas Ters

Marko Mihovilovic

Michael Schön

Johanna Hölbling

Ewald Srebotnik

Martina Brandner

Chularat Krongtaew

Marta Sut-Vejda

Michael Schon

Johanna Holbling

A process for the enzymatic regeneration of the redox cofactors NAD/NADH and NADP/NADPH in a one-pot reaction, wherein, as a result of at least two further enzymatically catalyzed redox reactions proceeding in the same reaction batch (product-forming reactions), one of the two redox cofactors accumulates in its reduced form and, respectively, the other one in its oxidized form, characterized in that a) in the regeneration reaction which reconverts the reduced cofactor into its original oxidized form, oxygen or a compound of general formula RC(O)COOH is reduced, and b) in the regeneration reaction which reconverts the oxidized cofactor into its original reduced form, a compound of general formula RCH(OH)Ris oxidized and wherein R, Rand Rin the compounds have different meanings.

Process for the enzymatic regeneration of redox cofactors

The present invention relates to a process for the conversion of D-glucose. The process according to the invention comprises a step a) in which part of the D-glucose is enzymatically oxidized to D-gluconic acid (lactone) and a portion of the D-glucose which is essentially equimolar thereto is reduced to D-sorbitol. In addition to D-glucose, D-fructose is also present in the reaction mixture of step a). The process comprises a further step b) in which D-sorbitol formed from the D-glucose in step a) is enzymatically oxidized to D-fructose.

Process for the conversion of sugars

Disclosed is a method for the isomerization of glucose by reduction to sorbitol and subsequent oxidation to fructose, in which the redox cofactors NAD/NADH and NADP/NADPH are regenerated in a one-pot-reaction, wherein one of the two redox cofactors is obtained in the reduced form thereof and the other redox cofactor in the oxidized form thereof as a result of at least two additional enzymatically catalyzed redox reactions (product forming reactions) taking place in the same reaction batch, wherein a) in the regeneration reaction, which transfers the reduced cofactor back to its originally oxidized form, oxygen or a compound of the general formula RC(O)COOH is reduced, and b) in the regeneration reaction, which transfers the oxidized cofactor back to its originally reduced form, a compound of the general formula RCH(OH)Ris oxidized, wherein R, Rand Rhave different meanings in the compounds, characterized in that a mixture of glucose and fructose is used as a starting material. Furthermore, the use of fructose thus produced in a method for producing furan derivatives is disclosed.

Method for the isomerisation of glucose

A method for the enzymatic production of D-fructose from D-glucose in a one-pot synthesis, wherein D-glucose is oxidized enzymatically to D-glucosone and D-glucosone is reduced enzymatically to D-fructose and the use of the D-fructose produced in this way for the production of furan derivatives.

Method for producing fructose

A process for the production of carbohydrate cleavage products, characterized by a combination of the measures that a lignocellulosic material is treated with an aqueous solution containing an alcohol, in particular a C-alcohol or a phenol, and having a pH-value of between 11.0 and 14.0 in order to cleave lignocellulose and separate cleavage products from the material, whereby a material enriched with cellulose and hemicellulose is obtained, and the obtained material enriched with cellulose and hemicellulose is treated with at least one carbohydrate-cleaving enzyme in order to obtain the carbohydrate cleavage products.

Process for the production of carbohydrate cleavage products from a lingnocellulosic material

A process for the production of furan derivatives from D-glucose, wherein

Process for the production of furan derivatives from glucose

A method for the preparation of lignin from lignocellulosic material by pulping with alcohol, in particular with a Calcohol, water and a base, in particular NaOH, at a temperature below 100&#xb0; C., in particular from 40&#xb0; C. to 90&#xb0; C., in particular from 50&#xb0; C. to 70&#xb0; C., characterized in that 3 to 12 parts of base, in particular 4 to 10 parts of base, in particular preferable 5 to 8 parts of base, are used per 100 parts of the dry lignocellulosic material to be pulped.

Preparation of lignin

A method for producing lignin derivatives from technical lignins by treatment with proteolytic enzymes (proteases), characterized in that the proteolytic treatment of the technical lignins significantly reduces the molar mass thereof.

Method for producing lignin derivatives

A method of obtaining an aqueous solution of an alkali acetate from lignocellulose, wherein lignocellulose is treated with a first aqueous extraction solution having a pH value in the range of 12 to 14 and a content of a Calcohol, in particular ethanol or isopropanol, in the range of 40% to 90%, in particular of from 75% to 85%, whereby a first aqueous solution of an alkali acetate is obtained, and wherein said first solution of an alkali acetate is used to treat additional lignocellulose in order to enrich additional acetate in said first solution.

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Vasoldsberg, Austria

Ortwin Ertl