Harvard College

The General Hospital Corporation

Oren Levy

Donald Elliot Ingber

Richard Novak

David Benson Chou

Arianna Rech

Viktoras Frismantas

Liliana S Teixeira Moreira Leijten

Yuka Milton

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

Bone marrow microfluidic devices and methods for preparing and using the same

Growing community of inventors

Brookline, MA, United States of America

Oren Levy