Gist-brocades N.v.

Genencor International, Inc.

Plant Genetic Systems N.v.

Plant Genetics System, Nv

Onno Misset

Jan Metske Van Der Laan

Wilhelmus J Quax

Johannes C Van Der Laan

Roelck A Cuperus

Ignace Lasters

Christiaan A Van Eekelen

Nadir Mrabet

Anne-Marie Lambeir

Herman B Lenting

Johan H Lensink

Leonardus J Mulleners

Leonardus Johannes Mulleners

Cornelis P Broekhuizen

Franciscus Josephus Cornelius Van Gastel

Erik Jan Baas

Johan H A Lensink

Christian A G Van Eekelen

Leonardus J S M Mulleners

Leonardus Johannes Sofie Marie Mulleners

Wilhelmus Johannes Quax

Johannes Cornelis Van Der Laan

Christiaan Albertus Van Eekelen

Johan Herman Lensink

Roelck Anneke Cuperus

Cornelis Petrus Broekhuizen

Franciscus Josephus Van Gastel

High alkaline serine protease

New proteolytic enzymes are provided exhibiting improved properties for application in detergents, especially laundry detergents. These enzymes are obtained by expression of a gene encoding a proteolytic enzyme having an amino acid sequence which differs at least in one amino acid from the wild type enzyme. Preferred enzymes are certain mutants derived from the serine protease of Bacillus nov. spec. PB92.

Protease muteins and their use in detergents

New mutant .beta.-lactam acylases are provided exhibiting altered substrate specificities. These .beta.-lactam acylases are obtained by expression of a gene encoding said .beta.-lactam acylase and having an amino acid sequence which differs at least in one amino acid from the wild-type .beta.-lactam acylase.

Mutated .beta.-lactam acylase genes

A method for selecting amino acid residues is disclosed which upon replacement will give rise to an enzyme with an altered pH optimum. The method is specific for metalloenzymes which are inactivated at low pH due to the dissociation of the metal ions. The method is based on altering the pK.sub.a of the metal coordinating ligands or altering the K.sub.ass for the metal binding. New glucose isomerases with an altered pH optimum are provided according to this method. These altered properties enable starch degradation to be performed at lower pH values.

Glucose isomerases with an altered pH optimum

Modified prokaryotic glucose isomerase enzymes with altered pH activity

PB92 serine protease muteins and their use in detergents

Recombinant genetic means for the production of serine protease muteins

Growing community of inventors

Delft, Netherlands

Onno Misset