Roche Sequencing Solutions, Inc.

Roche Diagnostics Gmbh

F. Hoffmann-la Roche Ag

Omid Khakshoor

Frank Bergmann

Meng Taing

Peter Crisalli

Dieter Heindl

Christoph Seidel

Aruna Ayer

Marshall Winston Porter

Hannes Kuchelmeister

Dieter Hiendl

Sara Kelley

Lars Hillringhaus

Seong-Ho Shin

Rongxin Nie

The disclosure relates to tagged nucleoside compounds comprising a nucleotide polyphosphate covalently attached to a tag, wherein the compound is a polymerase substrate and the polymer moiety is capable of entering a nanopore linked to the polymerase and thereby altering the flow of ions through the nanopore. The disclosure also provides methods for preparing the tagged nucleoside compounds and for their use as tags in nanopore-based nucleic acid detection and sequencing.

Tagged nucleoside compounds useful for nanopore detection

The present disclosure provides 3' protected nucleotides, including those 3&#x2032; protected nucleotides having a detectable tag. Systems and methods of sequencing nucleic acids using the 3&#x2032; protected nucleotides are also disclosed, such as the sequencing of a nucleic acid using a nanopore or the sequencing of a nucleic acid via sequencing-by-synthesis.

3' protected nucleotides

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Lathrop, CA, United States of America

Omid Khakshoor