Diachemix LLC

Her Majesty the Queen in Right of Canada, as Represented by the Canadian Food Inspection Agency

Her Majesty the Queen in Right of Canada, as Represented by the Minister of Agriculture and Agri-Food

Om P Surujballi

Michael E Jolley

Mohammad Sarwar Nasir

Anna Romanowska

Kathryn Irene Stilwell

Charlotte E Rigby

The present invention provides an assay for detection of-infected animals. A tracer, comprising a peptide ofprotein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific forThe tracer comprises a peptide ofprotein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific forto produce a detectable change in fluorescence polarization.

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The present invention provides an assay for detection of serum antibodies to. A tracer, comprising a carbohydrate antigen isolated fromthat is conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture is then measured. The presence of serum antibodies tois indicated by a fluorescence polarization value of the mixture that is higher than the fluorescence polarization value of a control. The present invention further provides a tracer for use in a fluorescence polarization assay for antibodies specific for. The tracer comprises a carbohydrate antigen isolated fromand conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific forto produce a detectable change in fluorescence polarization.

Rapid serological test for paratuberculosis using fluorescence polarization technology

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Nepean, Canada

Om P Surujballi