Orion Diagnostica Oy

Licentia Oy

Olli Heikki Laitinen

Henri Rainer Nordlund

Vesa Pekka Hytönen

Markku Sakari Kulomaa

Vesa Hytönen

Two circularly permuted avidin monomers arc designed. The circularly permuted monomers are fused and the resulting fusion peptides (dcAvd) form a pseudo-tetrameric dual-chain avidin, which is biologically active in biotin binding and shows similar structural characteristics as wild-type avidin. The dcAvd makes the development of dual-affinity avidins possible by allowing the adjustment of the ligand binding properties in the half of the binding sites differently than in the rest of the sites. The present invention provides further a single-chain avidin (scAvd) where two dcAvd-molecules are fused together via a linker to form a single polypeptide with four binding sites for biotin or other ligand.

Avidin mutants

The present invention relates to new chimeric mutants of avidin protein with improved properties, e.g. thermostability, better stability against proteolysis, better charge properties (for example lower pl) compared to native avidin and avidin-related proteins, AVRs. The chimeric avidin mutants comprise mutants where a region or regions in avidin are substituted by a corresponding region or regions from an AVR protein.

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Pirkkala, Finland

Olli Heikki Laitinen