Centre National De La Recherche Scientifique

Sorbonne Université

Université Pierre Et Marie Curie (paris 6)

Institut National De La Sante Et De La Recherche Medicale (inserm)

Technische Universitát Dresden

Inserm

Olivier Goureau

Jose-Alain Sahel

Deniz Dalkara

Jens Duebel

Sacha Reichman

José-Alain Sahel

Marusa Lampic

Marius Ader

Antoine Chaffiol

Marcela Garita-hernandez

The disclosure pertains to a defined cell culture medium for the expansion of human retinal progenitors, comprising or consisting of a nutrient medium, a SHH-pathway activator and a GSK3 inhibitor. To the use of the defined cell culture medium for the expansion of retinal progenitors, as well as to an in vitro method for expanding retinal progenitors, comprising: (i) placing a culture of human retinal progenitors in a defined cell culture medium as defined in claimsto; and (ii) culturing the cells in said defined cell culture medium.

Compositions and methods for efficient amplification of retinal progenitors cells

A method for in vitro obtaining human retinal progenitors, includes the steps of (i) placing an adherent culture of human pluripotent stem cells in a pro-neural medium; and (ii) maintaining this culture in the pro-neural medium until the appearance of pigmented cells and/or of neuroepithelial-like structures. Additional steps can be performed to obtain RPE cells and/or precursors of the neural retina.

Methods for obtaining retinal progenitors, retinal pigmented epithelial cells and neural retinal cells

Growing community of inventors

Paris, France

Olivier Goureau