Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Horiba Abx Sas

Iprasense Sas

Horiba Abx Sas / Horiba Ltd

Olivier Cioni

Thomas Bordy

Lionel Herve

Pierre Blandin

Cedric Allier

Sophie Morel

Camille Deforceville

Salim Boutami

Pierre Joly

Cédric Allier

Selimen Benahmed

Aurelien Daynes

Anais Ali-Cherif

Ondrej Mandula

Estelle Gremion

Sebastien Raimbault

Jean-Marc Dinten

Sébastien Raimbault

Aurélien Daynes

A method for characterizing a particle present in a sample, the sample lying between an image sensor and a light source and the sensor lying in a detection plane, includes illuminating the sample with the light source which emits an incident light wave propagating along a propagation axis, and acquiring an image of the sample with the sensor. The sensor is exposed to an exposure light wave. The image includes a plurality of elementary diffraction patterns each corresponding to one particle. The method also includes reconstructing a complex image representative of a complex amplitude of the light wave on a reconstruction surface passing through the sample, based on the acquired image; selecting a region of interest of the complex image corresponding to a particle of interest; forming an extracted image based on the region of interest; and characterizing the particle of interest depending on the extracted region of interest.

Method for characterising a particle on the basis of a hologram

A method for analyzing microorganisms arranged in a sample is provided, the sample including a viability marker to modify an optical property of the microorganisms in different ways depending on whether they are dead or alive, the method including illumination of the sample and acquisition of an image of the latter by an image sensor, the image sensor then being exposed to an exposure light wave; determining positions of different microorganisms from the acquired image; applying a propagation operator to calculate at least one characteristic value of the exposure light wave at each radial position and at a plurality of distances from the detection plane representing a change in the characteristic value between the image sensor and the sample; and identifying living microorganisms according to each profile.

Method for analysing microorganisms

The invention relates to a method for counting particles, particularly blood cells, in a sample, using a lensless optical imaging device. The sample is arranged between a light source and an image sensor. The sample is illuminated by a light source and an image is acquired by the image sensor, said image sensor being exposed to a light wave called an exposition wave. A digital propagation operator is applied to the acquired image so as to obtain a complex amplitude of the exposition wave according to a surface facing the image sensor. An image, called a reconstructed image, is formed from the modulus and/or the phase of said complex amplitude, on which image the particles to be counted appear in the form of regions of interest. The method then comprises a step of selecting the regions of interest corresponding to the particles to be counted.

Method for counting particles in a sample by means of lensless imaging

A device and method for forming an image of a sample includes illuminating the sample with a light source; acquiring a plurality of images of the sample using an image sensor, the sample being placed between the light source and the image sensor, no magnifying optics being placed between the sample and the image sensor, the image sensor lying in a detection plane, the image sensor being moved with respect to the sample between two respective acquisitions, such that each acquired image is respectively associated with a position of the image sensor in the detection plane, each position being different from the next; and forming an image, called the high-resolution image, from the images thus acquired.

Method for forming a high resolution image by lensless imaging

Method for observing a sample comprising the steps of (a) illuminating the sample using a light source, the light source emitting an incident light wave that propagates toward the sample along a propagation axis (Z); (b) acquiring, using an image sensor, an image of the sample, which image is formed in a detection plane; (c) forming a stack of images, called reconstructed images, from the image acquired in step (b), each reconstructed image being obtained by applying, for one reconstruction distance, a numerical propagation operator; and (d) from each image of the stack of images, computing a clearness indicator for various radial positions, each clearness indicator being associated with one radial position and with one reconstruction distance.

Method for observing a sample by lensless imaging, with a spatial dispersion in the sample taken into account

There is provided a device allowing a sample to be observed in a first mode, by lensless imaging using a first sensor. The first mode allows a first image to be obtained, on the basis of which a region of interest of the sample may be identified. The device then allows, via a relative movement, the region of interest to be analyzed using a more precise second mode and in particular using an optical system coupled to a second sensor.

Device for observing a sample and method for observing a sample

An object of the invention is an image sensor comprising a matrix of pixels, extending along a detection plane, and configured to form an image of an incident light wave propagating in a spectral band along a propagation axis, the image sensor being characterized in that it comprises a mask, formed by opaque elementary masks, extending parallel to the detection plane, between which there extend openings through which the incident light wave can propagate toward the detection plane, the matrix of pixels being divided into:

Image sensor for obtaining information relating to the phase of a light wave

A method for observing a sample includes illuminating the sample with a light source and forming a plurality of images, by an imager, the images representing the light transmitted by the sample in different spectral bands. From each image, a complex amplitude representative of the light wave transmitted by the sample is determined in a determined spectral band. The method further includes backpropagation of each complex amplitude in a plane passing through the sample, determining a weighting function from the back-propagated complex amplitudes, propagating the weighting function in a plane along which the matrix photodetector extends, updating each complex amplitude, in the plane of the sample, according to the weighting function propagated.

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Grenoble, France

Olivier Cioni