Conagen Inc.

Oliver Yu

Guohong Mao

Venkata Sai Prakash Chaturvedula

Hui Chen

Michael Batten

Jacob Edward Vick

Hongxue Wang

Jixiang Han

Provided herein are methods for making gamma lactones comprising reacting a carboxylic acid substrate with a heterologous cytochrome P450 (CYP450) protein with carboxylic acid 4-hydroxylase activity.

Biosynthetic production of gamma-lactones

The present disclosure relates to the use of beta-glucosidase to enhance the production efficiency of desired steviol glycosides, such as rebaudioside M (reb M).

Hydrolysis of steviol glycosides by beta-glucosidase

Disclosed is a steviol glycoside referred to as rebaudioside D2. Rebaudioside D2 has five &#x3b2;-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D2, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.

Non-caloric sweetener

A biosynthetic method of making a capsaicinoid including expressing a first gene product of CS/AT3/Pun1 in a cellular system, growing the cellular system in a medium, and collecting the capsaicinoid. The biosynthetic method further includes expressing a second gene product of ACS1 and expressing a third gene product of pAMT in the cellular system.

Methods of using capsaicin synthase for the microbial production of capsaicinoids

Disclosed is a steviol glycoside referred to as rebaudioside D3. Rebaudioside D3 has five &#x3b2;-D-glucosyl units connected to the aglycone steviol. Also disclosed are methods for producing rebaudioside D3, a UDP-glycosyltransferase fusion enzyme, and methods for producing rebaudioside D and rebaudioside E.

Growing community of inventors

Lexington, MA, United States of America

Oliver Yu