University of Münster

Oliver Spadiut

Diana Humer

Julian Ebner

Julian Quehenberger

David Wurm

The present invention provides a polypeptide having peroxidase activity and comprising an amino acid sequence having at least 70% sequence identity to SEQ ID NO: 3, wherein said amino acid sequence comprises at least one amino acid exchange compared to SEQ ID NO: 1, wherein said at least one amino acid exchange is an exchange of the amino acid P146 or of the amino acid N275 of SEQ ID NO: 1. The invention further relates to a nucleic acid molecule comprising a sequence encoding said polypeptide, an expression vector comprising said nucleic acid molecule, and a host cell comprising said expression vector. Moreover, methods for producing said polypeptide and compositions and kits comprising said polypeptides are provided.

Polypeptides with peroxidase activity

The present invention provides methods for producing a heme pe-roxidase from inclusion bodies (IBs) comprising the steps of: providing the heme peroxidase in the form of IBs, solubilizing said IBs, transferring said solubilized IBs into a refolding buffer to obtain a refolding mix, adding a heme cofactor to said refolding mix, wherein the addition of the heme cofactor to the refolding mix is distributed over a time period of at least 1 hour. The invention further provides methods for producing heme peroxidase products.

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Vienna, Austria

Oliver Spadiut