Leica Microsystems Cms Gmbh

Oliver Schlicker

Frank Sieckmann

Florian Fahrbach

Christian Schumann

Falk Schlaudraff

Kai Ritschel

Markus Schechter

An image processing apparatus for determining a focused output image in a passive autofocus system is configured to retrieve a set of input images and compute a baseline estimate for at least one input image. The baseline estimate represents image structures in the input image. The image structures have a length scale larger than a predetermined image feature length scale. The image processing apparatus is further configured to compute a set of output images, wherein each output image of the set of output images is computed based on one of a different input image of the set of input images and the at least one baseline estimate for the different input image and the at least one baseline estimate for a respective different input image. The image processing apparatus is further configured to determine one output image of the set of output images as the focused output image.

Image processing apparatus and method for use in an autofocus system

A microscope includes illumination optics for fluorescence excitation of point light sources of a sample, detection optics and a camera having a sensor. A density of the point light sources is kept low so as to minimize a crossover of point light sources that are behind or close to one another in each image captured by the camera. A means for subdividing a detection aperture into individual sub-apertures is provided in a beam path of the detection optics such that images generated by the individual sub-apertures on the sensor of the camera depict an object volume from different spatial directions.

Microscope and method for localizing fluorescent molecules in three spatial dimensions

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Wetzlar, Germany

Oliver Schlicker