Nmi Naturwissenschaftliches Und Medizinisches Institut an Der Universitat Tubingen

Chromotek Gmbh

Oliver Poetz

Larisa Yurlova

Kourosh Zolghadr

Tina Romer

Ulrich Rothbauer

Jaqueline Bogner

Andrea Buchfellner

Benjamin Ruf

Christian Linke-Winnebeck

Michael Metterlein

Markus Templin

Thomas Joos

Dieter Stoll

Fridolin Treindl

Anette Doettinger

Provided herein is a novel epitope that can be used as a tag in methods for rapid and effective characterization, purification, and subcellular localization of polypeptides of interest, which comprise the tag. The tag is specifically recognized by an epitope specific antibody, which can be used to detect, capture, quantify, and/or purify polypeptides of interest that are tagged with the epitope. Also provided is novel epitope specific antibody.

Epitope tag and method for detection and/or purification of tagged polypeptides

A method of detecting biomolecules present in a complex biological sample includes a) separating the biological sample into fractions according to at least one physical property of the biomolecules; and b) specifically detecting biomolecules present in the fractions using at least one solid-phase-based, immunological detection method including immobilizing the biomolecules from the individual fractions on microsphere populations specific for each fraction and distinguishable from one another.

Method for detecting biomolecules

Antibody, epitope tag and method for detection, capture and/or purification of tagged polypeptides

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Tübingen, Germany

Oliver Poetz