Metabolix, Inc.

Massachusetts Institute of Technology

Cj Cheiljedang Corporation

Yield10 Bioscience, Inc.

Other

Nuseed Nutritional US Inc.

Cj Research Center LLC

Oliver P Peoples

Frank Anthony Skraly

Anthony J Sinskey

Gjalt W Huisman

Kristi D Snell

David P Martin

Johan Van Walsem

Simon F Williams

Yossef Shabtai

Allen R Padwa

Thomas Martin Ramseier

Lara Madison

Jihong Tang

Nii Patterson

Rajendra K Krishnaswamy

Christopher L Mirley

Meghna Malik

Donald D Easson, Jr

Karen Bohmert-Tatarev

Laura Z Luo

Lauralynn Kourtz

Christopher W J McChalicher

Zhigang Zhang

Nirmala Sharma

Maurice M Moloney

Stephen Herbert Harris

Scott E Nichols

Larry R Beach

Yelena Kann

Luhua Zhong

Kevin A Sparks

Court A Saunders

Mariya Somleva

William R Farmer

Jixiang Han

Madana M R Ambavaram

David Boudreau

Jeffrey A Bickmeier

Maximillian T Follettie

Wolfgang Liebl

Venkatesh Bollina

Derek Samuelson

Venkata Tavva

Andrew Hertig

Max Senechal

Susan McAvoy

Mansoor Akhthar Basheer Ahmed

Julie Beaulieu

Sung Min Park

Bradley Garrison

Joseph Gredder

Stephanie Aquin

Catherine Morse

Himani Chinnapen

Anthony L Sinskey

Tillman U Gerngross

Yuanyuan Ji

Dirk Schweitzer

Matthew Martino

Claire Burkitt

Richard P Eno

Kieran Ryan

Ahmed Mansoor Akhthar Basheer

A transgenic land plant that expresses a polyhydroxy alkanoate synthase seed specifically, with cytosolic localization is disclosed. The plant includes a nucleic acid encoding the polyhydroxy alkanoate synthase and a seed-specific promoter operably linked to the nucleic acid. The seed-specific promoter drives expression of the polyhydroxy alkanoate synthase in cytosol of cells of seeds of the plant. The polyhydroxy alkanoate synthase includes a catalytic domain. The polyhydroxy alkanoate synthase does not include any sequence positioned to mediate translocation of the catalytic domain across any membrane of the cells, thereby resulting in the polyhydroxy alkanoate synthase being expressed seed specifically, with cytosolic localization.

Transgenic land plants that express a polyhydroxyalkanoate synthase seed specifically with cytosolic localization

A genetically engineered land plant that expresses a protein that has homology to a plant invertase inhibitor and/or a pectin methylesterase inhibitor and that increases seed yield with increased expression ('an ISY protein') is disclosed. The plant comprises a modified gene for the ISY protein. The ISY protein comprises one or more of an ISY protein ofcomprising SEQ ID NO: 2 or a fragment,homolog, or ortholog thereof. The modified gene comprises a promoter and a nucleic acid sequence encoding the ISY protein. The promoter is non-cognate with respect to the nucleic acid sequence encoding the ISY protein. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the ISY protein. A genetically engineered land plant that expresses an RNA that increases seed yield with increased expression also is disclosed.

Genetically engineered land plants that express an increased seed yield protein and/or an increased seed yield RNA

A genetically engineered land plant that expresses a plant CCP1-like mitochondrial transporter protein is provided. The genetically engineered land plant comprises a modified gene for the plant CCP1-like mitochondrial transporter protein. The plant CCP1-like mitochondrial transporter protein is an ortholog of CCP1 ofof SEQ ID NO: 1 derived from a source land plant. The plant CCP1-like mitochondrial transporter protein is localized to mitochondria of the genetically engineered land plant based on a mitochondrial targeting signal intrinsic to the plant CCP1-like mitochondrial transporter protein. The modified gene comprises (i) a promoter and (ii) a nucleic acid sequence encoding the plant CCP1-like mitochondrial transporter protein. The promoter is non-cognate with respect to the nucleic acid sequence. The modified gene is configured such that transcription of the nucleic acid sequence is initiated from the promoter and results in expression of the plant CCP1-like mitochondrial transporter protein.

Genetically engineered land plants that express a plant CCP1-like mitochondrial transporter protein

Gamma-butyrolactone ('GBL') and Gamma-hydroxybutyrate ('GHB') having a unique carbon footprint as defined by the percent modern carbon (pmc) are described herein. The percent modern carbon can be controlled by varying the amounts of biobased, renewable starting materials and petroleum-based starting materials to prepare GBL or GHB having a defined pmc or by preparing mixtures of GBL or GHB prepared from biobased renewable starting materials and GBL or GHB prepared from petroleum-based starting materials.

Production of salts of 4-hydroxybutyrate using biobased raw materials

Polymer systems comprising poly(vinyl chloride), a non-petroleum based filler, and a polyester additive, wherein the polymer system is highly filled, and articles made therefrom, are described. The high loading of filler (e.g., talc, calcium carbonate or a combination thereof) is achieved by incorporation of polyester additives such as polyhydroxyalkanoates. High filler loading without significant reduction of relevant material properties can be associated with cost savings and environmental benefits.

Highly filled polymer systems

A polyhydroxyalkanoate copolymer composition is provided. The composition comprises a plurality of polyhydroxyalkanoate copolymer molecules. The polyhydroxyalkanoate copolymer molecules (i) comprise 3-hydroxybutyrate monomers and 4-hydroxybutyrate monomers, (ii) have a monomeric molar percentage of 4-hydroxybutyrate monomers of 23.5 to 75%, and (iii) have a biobased content of &#x2265;80%. Also provided is a method of making a polyhydroxyalkanoate copolymer composition. The method comprises culturing an organism in the presence of one or more carbon raw materials under conditions under which (a) the one or more carbon raw materials are converted to 3-hydroxybutyryl-CoA and 4-hydroxybutyryl-CoA and (b) the 3-hydroxybutyryl-CoA and the 4-hydroxybutyryl-CoA are polymerized to form the polyhydroxyalkanoate copolymer molecules, thereby forming the composition. The organism has been genetically engineered to comprise particular enzymatic activities, and to not comprise other particular enzymatic activities. The one or more carbon raw materials, taken together, have a biobased content of &#x2265;80%.

Polyhydroxyalkanoate copolymer compositions and methods of making the same

Compositions of biobased polymer blends of polymers and a polyhydroxyalkanoate copolymer are described. In certain embodiments, the copolymer is a multiphase copolymer blend having one phase a glass transition temperature of about &#x2212;5&#xb0; C. to about &#x2212;50&#xb0; C. Methods of making the compositions of the invention are also described. Also articles, films and laminates made from the compositions are described.

Biobased rubber modified biodegradable polymer blends

Processes and methods for making biobased acrylic acid products including acrylic acid, acrylic acid oligomers, acrylic acid esters, acrylic acid polymers and articles from renewable carbon resources are described herein.

Renewable acrylic acid production and products made therefrom

Disclosed are organisms genetically engineered to make useful products when grown on ethanol as a carbon source. The organisms are genetically engineered to produce various useful products such as polyhydroxyalkanoates, diols, diacids, higher alcohols, and other useful chemicals.

Green process for producing polyhydroxyalkanoates and chemicals using a renewable feedstock

Compositions of biobased polymer blends of polymers of polybutylene succinate (PBS) or polybutylene-adipate-terephthalate (PBAT) and a polyhydroxyalkanoate copolymer are described. In certain embodiments, the copolymer is a multiphase copolymer blend having one phase a glass transition temperature of about &#x2212;5&#xb0; C. to about &#x2212;50&#xb0; C. Methods of making the compositions of the invention are also described. The invention also includes articles, films and laminates comprising the compositions.

Low glass transition polyhydroxyalkanoates for modification of biodegradable polymers

Transgenic plants, plant material, plant cells, and genetic constructs for synthesis of biopolymers, for example polyhydroxyalkanoates ('PHA') are provided. In one embodiment, the transgenic plants synthesize polyhydroxybutyrate ('PHB'). In one embodiment the transgenic plant encodes siRNA for one or more of the genes encoding enzymes for producing PHA. In a more preferred embodiment, the siRNA expression is under the control of an inducible regulatory element. In another embodiment, the transgenic plant contains transgenes that encode expression enzymes that will degrade the polymer. In a preferred embodiment, the expression of these enzymes is under the control of a germination specific, inducible, or minimal promoter. In another embodiment, the transgenic plant contains transgenes encoding enzymes that increase carbon flow for polymer synthesis. In a preferred embodiment, these transgenes encode enzymes that increase carbon flow in the Calvin Cycle.

Generation of high polyhydroxybutyrate producing oilseeds

Transgenic plants that produce high levels of polyhydroxybutyrate and methods of producing them are provided. In a preferred embodiment the transgenic plants are produced using plastid transformation technologies and utilize genes which are codon optimized. Stably transformed plants able to produce greater than 10% dwt PHS in tissues are also provided.

Stable, fertile, high polyhydroxyalkanoate producing plants and methods of producing them

DNA constructs and genetically engineered microbial strains constructed using these DNA constructs, which produce a nuclease enzyme with specificity for DNA and/or RNA, are provided. These strains secrete nuclease into the periplasm or growth medium in an amount effective to enhance productivity and/or recovery of polymer, and are particularly suited for use in high cell density fermentation processes. These constructs are useful for modifying microbial strains to improve production and recovery processes for polymers such as intracellular proteins, such as enzymes, growth factors, and cytokines; for producing polyhydroxyalkanoates; and for producing extracellular polysaccharides, such as xanthan gum, alginates, gellan gum, zooglan, hyaluronic acid and microbial cellulose.

Microbial strains and process for the manufacture of biomaterials

Methods and constructs for the introduction of multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway are provided. In one embodiment, the constructs contain two or more enzyme-encoding genes, each under the control of an inducible promoter and each with a polyadenylation signal. The constructs are used to produce transgenic plants, in which the expression of the enzymes are increased when a chemical inducing agent is applied, and a biosynthetic product of the series of enzymes encoded by the transgenes is produced. Constructs may be used which contain two or more enzyme-encoding genes under the control of one or more promoters activated by activator molecules or complexes expressed from a transgene or transgenes, which are themselves under the control of one or more inducible promoters and switched on following the external application of a chemical. The transgene or transgenes expressing the activator molecules or complexes may be included in the same construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. Alternatively, the transgene or transgenes expressing the activator molecules or complexes may be on a different construct from the construct containing multiple genes encoding enzymes in a multi-enzyme biosynthetic pathway. The activator molecule can be expressed using a constitutive promoter in an inactive form which is converted to the active form following application of the chemical inducing agent.

Chemically Inducible Expression of Biosynthetic Pathways

Microbial strains and processes for the manufacture of biomaterials

Growing community of inventors

Arlington, MA, United States of America

Oliver P Peoples