Abbott Diagnostics Scarborough, Inc.

Alere San Diego, Inc.

Cambridge Epigenetix Limited

Oliver Nentwich

Olaf Piepenburg

Catherine Jean Greenwood

Justin Davidson

Karthikeyan Kumaravadivelu

Maulik Vinod Patel

Alexander Schenk Zu Schweinsberg

Niall A Armes

Shankar Balasubramanian

Helen Rachel Bignell

Austin Derfus

Austin Matthew Derfus

Jens Fullgrabe

Nicolas James Walker

Michael Steward

Albert Vilella

Shirong Yu

Rita Santo San-Bento

Tobias Ost

Murray John Whyte

David Morley

Sidong Liu

Joanna Dawn Holbrook

Walraj Singh Gosal

A flu assay system including a sample module, a microfluidic nucleic acid amplification device, and an analyzer to facilitate fully automated nested recombinase polymerase amplification (RPA) on a sample delivered to the nucleic acid amplification device via the sample module. The assay includes providing a sample to a microfluidic device, and amplifying a target polynucleotide sequence in the sample. Amplifying the target polynucleotide sequence includes performing a first round of amplification on the sample to yield a first amplification product, and performing a second round of amplification on the first amplification product to yield a second amplification product. The second amplification product includes a smaller sequence completely contained within the first amplification product produced during the first round of amplification.

Automated nested recombinase polymerase amplification

A microfluidic device includes an inlet port configured to receive a sample, a first reaction chamber fluidically coupled to the inlet port, a first pump fluidically coupled to the inlet port, a second pump fluidically coupled to a mixing chamber, a metering channel fluidically coupled to the first reaction chamber and to the mixing chamber, and one or more second reaction chambers fluidically coupled to the mixing chamber. The first pump is configured to move fluid from the inlet port to the first reaction chamber and from the first pump to the inlet port. The second pump is configured to move fluid from the second pump to the mixing chamber, from the first reaction chamber to the mixing chamber, and from the mixing chamber to the one or more second reaction chambers.

Microfluidic devices and related methods

Provided herein are methods, systems, and compositions for determining a base in a polynucleotide. In various aspects, the methods, systems, and compositions presented herein are useful for performing 4-base, 5-base, or 6-base sequencing of polynucleotide molecules, for example, from liquid biopsy samples or wherein the base is a low frequency mutation.

Methods for analyzing nucleic acids

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Cambridge, United Kingdom

Oliver Nentwich