Slingshot Biosciences, Inc.

Zymergen Inc.

Topaz Biosciences, Inc.

Oliver Liu

Jeffrey Kim

Anh Tuan Nguyen

Jeremy Agresti

Jennifer Shock

William W Hwang

Zachariah Serber

Teresa Shock

Alexander Neckelmann

Xiao Yang

Eyal Akiva

Stephanie Leanne Brown Hendrix

Maritza Miller

Tom Hayon Eyles

Samuel Oteng-Pabi

Claus Pelikan

Ute Galm

Devin Scannell

Sangita Ganesh

Michael Sneddon

Maritza Duarte

Synthetic human cell mimic hydrogel particles and their use in cytometric or coulter device applications are described. The synthetic human cell mimic hydrogel particles described herein are selectively tunable to have at least one optical, volumetric, or capacitance property that is substantially similar to a corresponding optical, volumetric, or capacitance property of the human cell mimic hydrogel particle's natural biological cell counterpart.

Synthetic human cell mimic particle for cytometric or coulter device

The present disclosure relates to novel cell wall binding domains, enzymatically active domains, and chimeric cell wall hydrolases with anti-activity. The disclosure also relates to compositions comprising these, and uses thereof in the treatment of conditions associated with

Cell wall hydrolases targeting c. acnes

The present disclosure relates to novel chimeric cell wall hydrolases with anti-activity. The disclosure also relates to compositions comprising these chimeric cell wall hydrolases and uses thereof in the treatment of conditions associated withsp.

Recombinant cell wall hydrolases

Synthetic human cell mimic hydrogel particle for cytometric or coulter device

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to at least one optical property of a target cell. In this regard, the hydrogel particles provided herein, in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

Hydrogel particles with tunable optical properties and methods for using the same

Cytometric device hematology reference composition

The disclosure provides compositions and methods for producing natural products in microorganisms that are otherwise unexpressed, poorly expressed or poorly transcribed. In particular aspects, the disclosure provides compositions and methods for activating a silent gene or gene cluster with a bacteriophage and/orAntibiotic Regulatory Protein (SARP) transcription factor.

Compositions and methods for activating silent gene clusters

The present disclosure provides methods and systems for identifying natural product-encoding multi-gene clusters (MGCs). In some embodiments, the present disclosure also teaches methods for producing sequenced and assembled metagenomic libraries that are amenable to MGC search bioinformatic tools and techniques.

Metagenomic library and natural product discovery platform

The present disclosure provides methods and systems for identifying natural product-encoding multi-gene clusters (MGCs). In some embodiments, the present disclosure also teaches methods for producing sequenced and assembled metagenomic libraries that are amenable to MGC search bionformatic tools and techniques.

The disclosure presents a platform for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures and unique genetic amplification techniques, which enables access to a broad class of unknown microbial diversity and their resultant proteome. The disclosed insecticidal protein discovery platform (IPDP) can be computationally driven and is able to integrate molecular biology, automation, and advanced machine learning protocols. The platform will enable researchers to rapidly and accurately access the vast repertoire of untapped insecticidal proteins produced by uncharacterized and complex microbial environmental samples. Also presented herein are a group of newly discovered pore-forming toxins (PFT) from a rare class of insecticidal proteins, which were discovered utilizing the insecticidal protein discovery platform.

Insecticidal protein discovery platform and insecticidal proteins discovered therefrom

The disclosure is directed to an approach for discovering novel insecticidal proteins from highly heterogeneous environmental sources. The methodology utilizes metagenomic enrichment procedures that increase the representation of specific genera of bacteria, prior to deep sequencing, in order to access novel variants of insecticidal proteins. In aspects, the taught methods are useful for creating large genomic libraries enriched forsp. The enriched genomic libraries are useful for the discovery of novel cry toxin genes, which enable the identification of novel Cry proteins.

Method for creating a genomic library enriched forand identification of novel cry toxins

The present disclosure relates to compositions comprising a hydrogel particle with optical properties substantially similar to the optical properties of a target cell, and methods for their use.

Hydrogel particles with tunable optical properties

Hydrogel particles and their use in cytometric applications are described. The hydrogel particles described herein are selectively tunable to have at least one optical property substantially similar to the at least one optical property of a target cell. In this regard, the hydrogel particles provided herein in one aspect, are used as a calibration reagent for the detection of a target cell in a sample.

Growing community of inventors

San Francisco, CA, United States of America

Oliver Liu