Siemens Aktiengesellschaft

Siemens Healthcare Gmbh

International Business Machines Corporation

Siemens Healthcare Diagnostics Products Gmbh

Earlybio Gmbh

Oliver Hayden

Sandro Francesco Tedde

Lukas Richter

Matthias Ugele

Walter Gumbrecht

Oliver Schmidt

Maria Sramek

Michael Johannes Helou

Ali Kamen

Heike E Riel

Heinz Schmid

Walter Heinrich Riess

Manfred Stanzel

Noha Youssry El-Zehiry

Reiner Franz Schulz

Mikael T Bjoerk

Manfred Rührig

Gaby Marquardt

Tobias Rauch

Bogdan Georgescu

Frank Sauer

Saikiran Rapaka

Thomas Engel

Emanuel Loertscher

Anton Schick

Meinrad Schienle

Jens Fürst

Joachim Knoch

Roland Weiss

Ludwig Bär

Manfred Ruehrig

Helmut Eckert

Edgar Zaus

Daniela Seidel

Michael Vieth

Uwe Philippi

Jan Van Den Boogaart

Guido Zoli

Johannes Kern

Tobias Sterzl

Daniela Kühn

Mathias Reisbeck

Jon Ajuria Arregui

Francesco Arca

Gerald Warnat

The invention relates to a device, such as a digital holographic microscope, for detecting and processing a first full image of a measurement object, measured with a first offset, wherein an arrangement is provided for generating at least one further full image with at least one offset that differs from the first offset.

Multiple offset interferometer

The present invention relates to a method of detecting a possible infection of malaria in a patient using a digital optical microscope.

Specific malaria detection with digital holographic microscopy

A computer-implemented method for analyzing digital holographic microscopy (DHM) data for hematology applications includes receiving a DHM image acquired using a digital holographic microscopy system. The DHM image comprises depictions of one or more cell objects and background. A reference image is generated based on the DHM image. This reference image may then be used to reconstruct a fringe pattern in the DHM image into an optical depth map.

Auto-referencing in digital holographic microscopy reconstruction

The invention relates to a device for examining particles in a liquid to be examined, comprising a flow passage through which the liquid to be examined is moved. The flow passage has at least one inlet through which at least one sheath fluid flows into the flow passage such that the at least one sheath fluid forms at least one sheath flow in the flow passage. The device further comprises a wave generating device for piezoacoustically generating sound waves which propagate through the flow passage transversely to the flow direction of the liquid to be examined and form wave nodes on a monitoring plane such that particles to be examined of the liquid to be examined are moved onto the monitoring plane and accumulate thereon on the basis of the pressure effect of the sound waves in the transverse direction.

Flow cell for analyzing particles in a liquid to be examined

The invention relates to a method and a corresponding arrangement for sequencing at least two biopolymers (), wherein for each biopolymer () a sequence signal is picked up by a respective measured variable pickup on the basis of the sequence of the biopolymer (), the sequence signals are transferred to a shift register () and buffer-stored therein, the buffer-stored sequence signals are transferred from the shift register () sequentially to an evaluation device () and evaluated therein. Each sequence signal is preferably produced here by means of a nanopore arrangement (). A corresponding sequencing arrangement () has the measured variable pickups and the shift register () integrated in it, preferably in an electrical circuit, that is to say on a sensor array, for example. Each sequence signal can be amplified here by a preamplifier () prior to transfer to the shift register (). The transfer of the output signal (A) to the evaluation device () can comprise the amplification of the signal by an output amplifier () and/or at least one EMCCD stage ().

Sequencing biopolymers

The present invention relates to an improved method for marker-free detection of a cell type of at least one cell in a medium using microfluidics and digital holographic microscopy, as well as a device, particular for carrying out the method.

High accuracy 5-part differential with digital holographic microscopy and untouched leukocytes from peripheral blood

A flexible, thin, elongated band is used as a substrate. Similarly to a magnetic tape, the band is unwound from a feed reel and is transported past an outlet opening of a receptacle containing the cells such that the cells are poured onto the band. Subsequently, the band containing the cells applied thereon is wound onto a take-up reel. The take-up reel is fixed on a drive shaft which can be rotated by a drive mechanism. The rotation thus achieved has the effect that the band is unwound, transported past the outlet opening and finally wound up. In addition, spacers are provided at the upper surface of the band in order to prevent the contacting of radially adjacent sections of the band containing the cells in a wound-up state.

Arrangement, substrate and method for preparing a cell sample

A technique is presented for aligning, in a desired region within a flow chamber of a flow cell, a non-spherical biological entity carried in a sample. The flow chamber has a rectangular cross-section. A bottom flow input module, a top flow input module and a sample input module provide a viscoelastic first fluid, a second viscoelastic fluid, and the sample, respectively, to the flow chamber. The first and the second viscoelastic fluids laminarly flow along a bottom and a top wall of the flow chamber and the sample laminarly flows sandwiched between them. By controlling rate of flow of the first and/or the second viscoelastic fluids the sample flow, and thus the non-spherical biological entity, is focused in the desired region. A gradient of sheer within the sample flow set up due to the first and second viscoelastic fluids orients the non-spherical biological entity in the desired region.

Aligning a non-spherical biological entity in a sample flow using ambient viscoelastic fluid flows

The invention relates to a cartridge () for a magnetic flow cytometer, mainly extending in a x-y-plane, with an inlet () for injecting a sample () into the cartridge (), a blister () for a buffer solution () with magnetic markers to mark pregiven particles (') of the sample (), an outlet, and a fluid channel (), the fluid channel () comprising a first part that connects the inlet () with the blister () and a second part that connects the first part with the outlet, wherein the second part of the fluid channel () comprises an enrichment zone () with mechanical guiding structures to focus marked particles (&#x2032;) of the sample () in a predetermined subsection of the fluid channel () and a measuring zone () between the enrichment zone () and the outlet, the measuring zone () comprising a magnetic field sensor () in the predetermined subsection of the fluid channel () in order to provide simplified and accelerated means for measuring particles, in particular concentrations of particles, of a sample.

Cartridge for a magnetic flow cytometer, a magnetic flow cytometer, and method for analysing a sample with such a cartridge

An in vitro method for the marker-free determination of a cell type of a cell in a biological sample is disclosed, a microscopic device being configured to detect a height profile of the cell with respect to a carrier plate. An embodiment of the method performed by a cell analysis device, include: determining a cell compartment of the cell on the basis of the detected height profile, determining a predetermined quantitative cell feature on the basis of the determined cell compartment, and determining the cell type of the cell on the basis of the determined quantitative cell feature. A correspondingly designed cell analysis device and a corresponding microscopic device are also disclosed.

In vitro method for the label-free determination of a cell type of a cell

An assembly for nucleic acid sequencing by tunnel current analysis has at least two electrically conductive particles having a diameter from 1 nm to 100 nm and at least two electrically insulating particles having a diameter from 1 nm to 100 nm. The particles are in particular spherically shaped. The assembly also has at least two first electrodes for contacting the electrically conductive particles and a substrate on which the first electrodes and the particles are arranged. The four particles are arranged substantially in a planar square. The conductive particles lie diagonally opposite each other, and the insulating particles lie diagonally opposite each other. The gap between the four particles is used as a solid-state nanopore for nucleic acid sequencing.

Assembly for nucleic acid sequencing by means of tunnel current analysis

An assembly and a method are disclosed for analyzing nucleic acid sequences by way of so-called sequencing-by-synthesis. According to an embodiment of the invention, a chemical substance group that is released when a nucleotide bonds to a nucleic acid sequence to be sequenced is detected. The reagents are applied by way of a spraying device to a sensor that detects the released substance group. This has the advantage that no lateral flow occurs. The rate of false-negative and false-positive results is significantly reduced. Furthermore, a small amount of the reagent is sufficient to completely wet the sensor. Filling of the supply and discharge lines as for a flow cell is not necessary.

Assembly and method for analyzing nucleic acid sequences by way of so-called sequencing-by-synthesis

The invention relates to a device and a method for fluidic cell guidance for flow cytometry or analyte enrichment. This allows magnetically marked analytes, in particular cells (), to be dynamically enriched and individually detected in the flow from a sample, in particular magnetoresistively. For cell guidance, guiding ridges () are arranged in a flow channel (), and so, in addition to a magnetic enrichment force () and the shearing force of the flow (), a deflecting force () caused by the fluidic obstacles () also acts on the cells () to be detected.

Fluidic cell guidance for flow cytometry

The invention relates to a method for detecting ainfection in a patient blood sample, wherein a differential analysis of the polymorphonuclear neutrophil granulocytes in the sample is performed, and the distribution of the cell volume and the cell density, the number of thrombocytes in the sample, and the distribution of the cell density of the thrombocytes in the sample is determined.

Method for detecting a plasmodium infection

The embodiments relate to a device and to a method for concentrating and detecting cells in flowing media, in particular magnetically marked cells in complex media such as blood. For this purpose, at least one magnet is used, said magnet being coupled to at least one magnetoresistance. In the method the cells are concentrated on a magnetoresistor by the least one external magnetic field having a pulsed operation.

Device and method for concentrating and detecting magnetically marked cells in laminarly flowing media

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Herzogenaurach, Germany

Oliver Hayden