Stemcell Technologies Inc.

Other

Oliver Egeler

Steven M Woodside

Jason Everett Dowd

Gary Dossantos

Jason Dowd

A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 90 degrees and less than 180 degrees.

Meniscus reducing member

A meniscus reducing member for use in a vessel for containing a liquid may include a physical surface feature overlying at least a portion of an interior surface of the vessel. The physical surface feature may have first and second inner surfaces that are generally parallel and at least a third surface extending between the first and second surfaces. The first inner surface, second inner surface and third surfaces may be configured to physically alter a receding contact angle between the liquid and the physical surface feature. A coating material may be applied to at least one of the surfaces of the physical surface feature to chemically alter the receding contact angle between the liquid and the coated surface whereby the receding contact angle formed between the liquid and the meniscus reducing member is between about 75 degrees and 110 degrees.

A cell culture device comprises a well. A plurality of microwells are within the well, and a first common fluid volume is within the well above the microwells. A set of sub-microwells are within each microwell, and a second common fluid volume is within each microwell above the set of sub-microwells.

Cell culture device

A meniscus reducing member for use in a vessel for containing a liquid including a surface feature overlying at least a portion of an interior surface of the vessel. The surface feature includes at least two surfaces for contacting the liquid that cooperate to reduce a width of a meniscus formed at an interface between the liquid and the surface feature by physically altering a contact angle between the liquid and the surface feature.

Cell culture vessels for meniscus reduction with aqueous solutions

The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution.

Growing community of inventors

Vancouver, Canada

Oliver Egeler