Cargill Inc.

Novozymes A/s

Genencor International, Inc.

Danisco US Inc.

Olga V Selifonova

Volker Schellenberger

Gregory Marshall Whited

Holly J Jessen

Steven John Gort

Ravi R Gokarn

Wolfgang Buckel

Thorsten Selmer

Hans H Liao

Katherine D Collier

Donald Eugene Trimbur

Amy Deming Liu

Maria R Diaz-Torres

Thomas B Morrison

Amy D Liu

Edwin Lee

Nicholas Leiva

Adam C Kean

Trimbur E Donald

Whited M Gregory

Holly Jean Jessen

Edwin W Lee

Methods and materials related to producing 3-HP as well as other organic compounds are disclosed. Specifically, isolated nucleic acids, polypeptides, host cells, and methods and materials for producing 3-HP and other organic compounds are disclosed.

3-hydroxypropionic acid and other organic compounds

The present disclosure provides novel beta-alanine/alpha ketoglutarate aminotransferase nucleic acid and protein sequences having increased biological activity. Also provided are cells containing such enzymes, as well as methods of their use, for example to produce malonyl semialdehyde and downstream products thereof, such as 3-hydroxypropionic acid and derivatives thereof.

Beta-alanine/alpha-ketoglutarate aminotransferase for 3-hydroxypropionic acid production

An evolvable production strain ofexhibiting continuous or high level expression during protein evolution is described. An evolvedpstS promoter facilitates screening and production of secreted proteins.

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Methods of using beta-alanine/pyruvate aminotransferase to produce 3-hydroxypropionic acid and derivatives thereof, from beta-alanine, are disclosed. Cells and recombinant nucleic acids that can be used to practice the methods are also disclosed.

Production of 3-hydroxypropionic acid using beta-alanine/pyruvate aminotransferase

Alanine 2,3-aminomutase sequences are disclosed, as are cells having alanine 2,3-aminomutase activity and methods of selecting for such cells. Methods for producing beta-alanine, pantothenate, 3-hydroxypropionic acid, as well as other organic compounds, are disclosed.

Nucleic acid molecules encoding alanine 2,3-aminomutases

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Plymouth, MN, United States of America

Olga V Selifonova