Basf Plant Science Gmbh

Basf Plant Science Company Gmbh

Rothamsted Research Limited

Iacr-rothamsted

The University of Bristol

Rothamsted Experimental Station

Olga Sayanova

Johnathan A Napier

Richard Haslam

Jörg Bauer

Toralf Senger

Thorsten Zank

Sten Stymne

Laurent Marty

Jenny Lindberg Yilmaz

Ruiz Lopez Noemi

Jonathan A Napier

Monica Venegas Caleron

Jorg Bauer

Ernst Heinz

Ulrich Zähringer

Baoxiu Qi

Colin M Lazarus

Royah Vaezi

Joerg Bauer

Frederic Beaudoin

Mary Hamilton

Noemi Ruiz-Lopez

Noemi Ruiz Lopez

The invention provides isolated nucleic acid molecules which encode novel fatty acid desaturases and elongases from the organism. The invention also provides recombinant expression vectors containing desaturase or elongase nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g. arachidonic acid (ARA), eicosapentaenoic acid (EPA) or docosahexaenoic acid (DHA).

Uses of novel fatty acid desaturases and elongases and products thereof

A recombinantplant or cell comprising one or more polynucleotides encoding a &#x394;6-desaturase, a &#x394;6-elongase and a &#x394;5-desaturase operably linked with one or more regulatory sequences.

Production of omega-3 long chain polyunsaturated fatty acids

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucleotides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

Acyltransferases and uses thereof in fatty acid production

The invention relates to genetically modified organisms with enhanced production of omega-3 long chain polyunsaturated fatty acids.

Transgenic microalgae with increased production of at least one omega-3 long chain polyunsaturated fatty acid

Fatty acid desaturases and elongases and uses thereof

The present invention relates to the recombinant manufacture of polyunsaturated fatty acids. Specifically, it relates to acyltransferase polypeptides, polynucleotides encoding said acyltransferases as well as vectors, host cells, non-human transgenic organisms containing said polynucletides. Moreover, the present invention contemplates methods for the manufacture of polyunsaturated fatty acids as well as oils obtained by such methods.

The invention provides isolated nucleic acid molecules which encodes a novel fatty acid nECR. The invention also provides recombinant expression vectors containing nECR nucleic acid molecules, host cells into which the expression vectors have been introduced, and methods for large-scale production of long chain polyunsaturated fatty acids (LCPUFAs), e.g., ARA, EPA and DHA.

Fatty acid elongation components and uses thereof

The present invention relates to a polynucleotide fromwhich codes for a desaturase and which can be employed for the recombinant production of polyunsaturated fatty acids. The invention furthermore relates to vectors, host cells and transgenic nonhuman organisms which comprise the polynucleotide according to the invention, and to the polypeptides encoded by the polynucleotide. The invention furthermore relates to antibodies against the polypeptide according to the invention. Finally, the invention also relates to production methods for the polyunsaturated fatty acids and for oil, lipid and fatty acid compositions and to their use as drugs, cosmetics, foodstuffs, feedstuffs, preferably fish food, or food supplements.

Desaturase and method for the production of polyunsaturated fatty acids in transgenic organisms

The present invention relates to an improved method for the specific production of unsaturated &#x3c9;-3 fatty acids and a method for the production of triglycerides having an increased content of unsaturated fatty acids, in particular &#x3c9;-3 fatty acids having more than three double bonds. The invention relates to the production of a transgenic organism, preferably a transgenic plant or a transgenic microorganism, having an increased content of fatty acids, oils or lipids having &#x394;6 double bonds due to the expression of a &#x394;6-desaturase from Primulaceae. The invention additionally relates to expression cassettes containing a nucleic acid sequence, a vector and organisms containing at least one nucleic acid sequence or an expression cassette. The invention further relates to unsaturated fatty acids and triglycerides having an increased content of unsaturated fatty acids and use thereof.

Delta 6 desaturases from primulaceae, expressing plants and PUFA-containing oils

The present invention relates to a new process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a &#x394;-12-/&#x394;-15-desaturase, &#x394;-9-elongase, &#x394;-8-desaturase and a &#x394;-5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular &#x3c9;-3 and &#x3c9;-6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid, oils or lipids containing C- or C-fatty acids with a double bond in position5, 8, 9, 11, 12, 14, 15 or 17 of the fatty acid produced, respectively due to the expression of the &#x394;-12-/&#x394;-15-desaturase, of the &#x394;-9-elongase, of the &#x394;-8-desaturase and of the &#x394;-5-desaturase in the plant. The expression of the inventive &#x394;-12-/&#x394;-15-desaturase leads preferably to linoleic acid and linolenic acid as products having a double bond in the position9, 12 and 15 of the fatty acid. The invention additionally relates to specific nucleic acid sequences encoding for proteins with &#x394;-12-/&#x394;-15-desaturase-, &#x394;-9-elongase-, &#x394;-8-desaturase- or &#x394;-5-desaturase-activity, nucleic acid constructs, vectors and transgenic plants containing said nucleic acid sequences.

Process for the production of arachidonic acid and/or eicosapentaenoic acid

The invention relates to nucleic acid derived fromwhich encodes a 9-elongase, a &#x394;8-desaturase and a &#x394;5-desaturase enzyme. All of the coding sequences can be transcribed as a single transcript.

Elongase and uses thereof

The present invention relates to a new process for the production of arachidonic acid and/or eicosapentaenoic acid in plants through the co-expression of a &#x394;-12-/&#x394;-15-desaturase, &#x394;-9-elongase, &#x394;-8-desaturase and a &#x394;-5-desaturase and a process for the production of lipids or oils having an increased content of unsaturated fatty acids, in particular &#x3c9;-3 and &#x3c9;-6 fatty acids having at least two double bonds and a 18 or 20 carbon atom chain length. Preferably the arachidonic acid and eicosapentaenoic acid are produced in at least a 1:2 ratio. The invention furthermore relates to the production of a transgenic plants, preferably a transgenic crop plant, having an increased content of arachidonic acid and/or eicosapentaenoic acid, oils or lipids containing Ci&#x3b2;- or C20-fatty acids with a double bond in position &#x394; 5, 8, 9, 11, 12, 14, 15 or dffthe fatty acid produced, respectively due to the expression of the &#x394;-12-/&#x394;-15-desaturase, of the &#x394;-9-elongase, of the &#x394;-8-desaturase and of the &#x394;-5-desaturase in the plant. The expression of the inventive &#x394;-12-/&#x394;-15-desaturase leads preferably to linoleic acid and linolenic acid as products having a double bond in the position &#x394; 9, 12 and 15 of the fatty acid. The invention additionally relates to specific nucleic acid sequences encoding for proteins with &#x394;-12-/&#x394;-15-desaturase-, &#x394;-9-elongase-, &#x394;-8-desaturase- or &#x394;-5-desaturase-activity, nucleic acid constructs, vectors and transgenic plants containing said nucleic acid sequences.

The present invention relates to the production of &#x3b3;-linolenic acid (18:3) or stearidonic acid (18:4) or &#x3b3;-linolenic acid (18:3) and stearidonic acid (18:4) in transgenic plants of the family Brassicaceae, where the transgenic plants comprise at least 10% by weight of oleic acid based on the total fatty acid content and, as the result of the activity of the &#x394;6-desaturases used in the method, have an increased &#x394;6-C18-fatty acid content. The invention furthermore relates to novel nucleic acid sequences which code for the &#x394;6-desaturases used in the method, gene constructs comprising these nucleic acid sequences, a vector and transgenic plants comprising at least one nucleic acid sequence or a gene construct.

Growing community of inventors

St. Albans, United Kingdom

Olga Sayanova