Lumora Limited

Lumor Limited

Olga Gandelman

Laurence Carlo Tisi

Cathal Joseph McElgunn

Guy Kiddle

James Augustus Henry Murray

Victoria Louise Church

James Augustus Murray

Cathal Mcelgunn

Victoria Louise Church Armstrong

The invention is in the field of nucleic acid amplification, hi particular, methods are described which utilize stem primers that improve the rapid and specific amplification of a test sample.

Stem accelerated isothermal nucleic acid amplification technology

A method for determining the presence and/or amount of a first polynucleic acid in a sample comprising subjecting the sample to nucleic acid amplification in which product is detectable by the presence of a signal generated by polynucleic acid formation from the first polynucleotide characterised in that the nucleic acid amplification reaction is conducted, in the same reaction vessel, with a predetermined amount of a second polynucleic acid which is subjected to nucleic acid amplification, the product of which is detectable by the presence of the same signal generated by polynucleic acid formation from the second polynucleotide as that generated by polynucleic acid formation from the first polynucleotide and wherein the product of the second polynucleic acid is produced with different reaction kinetics from the product of the first polynucleic acid such that the second polynucleic acid acts as an internal control for the method.

Nucleic acid amplification

A method for determining the amount of template nucleic acid present in a sample comprising the steps of: i) bringing into association with the sample all the components necessary for nucleic acid amplification, and all the components necessary for a bioluminescence assay for nucleic acid amplification and subsequently: ii) performing the nucleic acid amplification reaction; iii) monitoring the intensity of light output from the bioluminescence assay; and iv) determining the amount of template nucleic acid present in the sample.

Method for determining the amount of template nucleic acid present in a sample

Growing community of inventors

Cambridge, United Kingdom

Olga Gandelman