The University of British Columbia

Oleh Petriv

Anupam Singhal

Veronique Lecault

Carl Lars Genghis Hansen

Adam Keith White

William Bowden

Leo Wu

Georgia Russell

Darek Sikorski

Dan Da Costa

Tim Leaver

Carl L G Hansen

Daniel Jay Da Costa

Kevin Albert Heyries

Marketa Ricicova

Hans Zahn

Brad Nelson

Michael VanInsberghe

Julie Nielsen

Kathleen Lisaingo

Michael Vanlnsberghe

Carl L Hansen

Carl Lars Hansen

Methods and devices are provided herein for identifying a cell population comprising an effector cell that exerts an extracellular effect. In one embodiment the method comprises retaining in a microreactor a cell population comprising one or more effector cells, wherein the contents of the microreactor further comprise a readout particle population comprising one or more readout particles, incubating the cell population and the readout particle population within the microreactor, assaying the cell population for the presence of the extracellular effect, wherein the readout particle population or subpopulation thereof provides a direct or indirect readout of the extracellular effect, and determining, based on the results of the assaying step, whether one or more effector cells within the cell population exerts the extracellular effect on the readout particle. If an extracellular effect is measured, the cell population is recovered for further analysis to determine the cell or cells responsible for the effect.

Microfluidic devices and methods for use thereof in multicellular assays of secretion

Microfluidic devices are provided for trapping, isolating, and processing single cells. The microfluidic devices include a cell capture chamber having a cell funnel positioned within the cell capture chamber to direct a cell passing through the cell capture chamber towards one or more a cell traps positioned downstream of the funnel to receive a cell flowing. The devices may further include auxiliary chambers integrated with the cell capture chamber for subsequent processing and assaying of the contents of a captured cell. Methods for cell capture and preparation are also provided that include flowing cells through a chamber, funneling the cells towards a cell trap, capturing a predefined number of the cells within the trap, interrupting the flow of cells, flowing a wash solution through the chamber to remove contaminants from the chamber, and sealing the predefined number of cells in the chamber.

Microfluidic cell trap and assay apparatus for high-throughput analysis

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Vancouver, Canada

Oleh Petriv