Phage Biotechnology Corporation

Oleg Okunev

Vitaliy A Kordyum

Olena Deryabina

Pavlo Gilchuk

Dmitro Irodov

A bacterial high-expression system which is applicable for simultaneous screening of large numbers of recombinant clones from combinatorial antibody libraries is disclosed. The method pertains to screening of single chain antibodies from libraries expressed in the periplasm ofby secretion. By this approach, approximately 10clones can be screened in a single round. After screening, the clones, which express the recombinant antibodies to the desired antigen, can be directly used for production of large quantities of antibodies from microorganism culture. The system is especially attractive for fast screening of antibody libraries from a hybridoma source. A refolding method for the large-scale production of biologically active scFv-6 his proteins from bacterial inclusion bodies is also disclosed.

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Kyiv, Ukraine

Oleg Okunev