Olympus Corporation

Olaf Lehman

Karsten Henco

Manfred Eigen

Michael Stuke

Wolfgang Simm

Rudolf Rigler

Ulo Mets

Jerker Widengren

Michael Brinkmeyer

A Method for identifying one or a small number of molecules, especially in a dilution of &#x2266;1 &#x3bc;M, using laser excited FCS with measuring times &#x2266;500 ms and short diffusion paths of the molecules to be analyzed, wherein the measurement is performed in small volume units of preferably &#x2266;10l, by determining material-specific parameters which are determined by luminescence measurements of molecules to be examined. The device which can be preferably used for performing the method according to the invention is a per se known system of microscope optics for laser focusing for fluorescence excitation in a small measuring compartment of a very diluted solution and for imaging the emitted light in the subsequent measurement through confocal imaging wherein at least one system of optics with high numerical aperture of preferably &#x2267;1.2 N.A. is employed, the light quantity is limited by a confocally arranged pinhole aperture in the object plane behind the microscope objective, and the measuring compartment is positioned at a distance of between 0 and 1000 &#x3bc;m from the observation objective.

Method and a device for the evaluation of biopolymer fitness

Growing community of inventors

Gottingen, Germany

Olaf Lehman