Ge Healthcare Bio-Sciences Ab

Cytiva Bioprocess R&d Ab

Ge Healthcare Bioprocess R&d Ab

Cytiva Sweden Ab

Puridify Ltd.

Ola Lind

Jean-Luc Maloisel

Ronnie Palmgren

Bjorn Noren

Klaus Gebauer

Nils Norrman

Tobias Soderman

Ingrid Drevin

Gustav Jose Rodrigo

Lars C Andersson

James Van Alstine

Eric Faldt

Andreas Torbjorn Lundin

Kajsa Stridsberg-Friden

Tobias Söderman

Jamil Shanagar

Johan Fredrik Avallin

Marc Hummersone

Raf Lemmens

Jan Berglof

Matthew Townsend

Karl Axel Jakob Liderfelt

Elisabeth Hallgren

Ida Eklind

Karl Liderfelt

Bjorn Johan Lundgren

Gunnar Malmquist

Ian Scanlon

Johan Avallin

Anita Solbrand

Niklas Jungnelius

Jesper Hedberg

Tania Ahmad

Ulf Segerbo

Michael Katzke

Anna Arefalk

Hamid Houshmand

Helena Widegren

Jon Lundqvist

Jimmy Hedin Dahlström

Sara Häggblad Sahlberg

Björn Lundgren

Björn Norén

Bjoern Lundgren

Tobias E SÖDERMAN

Kajsa Stridsberg-FRIDÉN

The present invention relates to a preparative chromatography system () and a chromatography process () adapted to repetitive cycling of chromatography volumes. The system () comprises at least two upstream pumps () and separate flow paths () from process liquid sources to the chromatography device (). The system () is arranged to prime one flow path () with one process liquid while providing another process liquid to the chromatography device and thereby minimizing the hold-up volume of the system ().

Preparative chromatography system and method for chromatography separations

The invention discloses an immunoglobulin-binding magnetic bead, comprising a porous matrix and one or more magnetic particles embedded in said matrix, wherein said matrix comprises a porous polymer and at least 10 mg/ml Fc-binding proteinaceous ligands covalently coupled to said porous polymer.

Magnetic immunoglobulin-binding particles

A separation system, a method in a separation system and an elution arrangement to be provided in a separation system for separating a biomolecule from a cell culture are provided. The method comprises the steps of: &#x2014;providing a feed from a cell culture () comprising said biomolecule to a magnetic separator () and providing to the magnetic separator magnetic beads comprising ligands capable of binding this biomolecule; &#x2014;separating by the magnetic separator said magnetic beads with bound biomolecules from the rest of the feed; &#x2014;forwarding said magnetic beads as a slurry with an added buffer to an elution cell (); &#x2014;eluting the bound biomolecules in the elution cell.

Method and system for separating biomolecules

The present invention relates to a method for removal of large contaminants, such as virus, in a chromatographic process for purification of a target molecule, preferably monoclonal antibodies, mAbs, by using a specifically designed chromatographic bead having a thin outer layer and a core functionalized with a ligand adsorbing the mAbs or parts thereof.

Chromatography method

The invention discloses a separation matrix for purification of biomacromolecules, comprising a plurality of particles () having a core region () and a shell region (), wherein: a) said shell region is accessible to a target biomacromolecule; b) said core region is less accessible to the target biomacromolecule than the shell region; and c) the core region comprises a grafted polymer comprising residues of at least one polymerizable monomer.

Chromatography matrix

The invention discloses a separation matrix with a porous solid support and a plurality of polyvinylpyrrolidone (PVP) polymer chains covalently attached to the solid support. The polyvinylpyrrolidone polymer chains are either vinylpyrrolidone homopolymer chains or copolymer chains which comprise at least 70 mol % vinylpyrrolidone monomer residues and less than 2 mol % negatively charged monomer residues.

Stabilization of fermented beverages

The invention provides a packing method for high efficiency chromatography columns starting from dry swellable particles, as well as columns packed by the method and the use of the columns in separation of biomolecules. In the packing method, an amount of dry swellable particles sufficient to give a swollen volume in a liquid of about 105-120% of the column chamber volume is transferred to the column, the column is closed and the liquid is provided to the column.

Method for dry packing chromatography columns

The present invention relates to a method for sterilization of different materials, especially sensitive material, such as chromatographic media with sensitive ligands. The method for sterilization of a chromatographic separation medium comprises exposing the separation medium to pressurized steam at a temperature of between about 121&#xb0; C. and about 135&#xb0; C.

Sterilization method

The present invention is relates to method for production of a biomolecule adsorbent with a pentadentate ligand with very strong chelating properties, and to methods for use thereof for, for example, protein purification, detection or binding. The biomolecule adsorbent comprises the formula: wherein Q is a carrier, S is a spacer, L is an amide linkage, X is COOH, and n=2 or 3.

Method for preparation of a biomolecule adsorbent

The present invention relates to liquid clarification. More closely, the invention relates to beverage clarification, such as reduction of colloidal (not microbial) haze-causing substances in beer or related beverages such as wine, juices, flavorings etc. The method of the invention uses a hydrophilic surface for adsorption of haze-forming substances by hydrogen bonding interaction properties between the surface and the haze-forming substances.

Liquid clarification

The present invention relates to a method of sterilizing a chromatography column which is packed with a separation matrix, which method comprises passing a superheated aqueous liquid through the packing of the column to obtain at least one substantially sterile packed chromatography column. The method may also comprise a preceding heating. In the most advantageous embodiment, the superheated aqueous liquid is passed in a substantially uniform flow through the packing of the column, resulting in a sterility of the packed column.

Method of sterilization

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Uppsala, Sweden

Ola Lind