The Institute of Physical and Chemical Research

Shin-estu Chemical Co., Ltd.

Okmi Park

Jun Watanabe

Isao Endo

Tohru Chiba

Hiroshi Miyoshi

According to the method for preparing chitosan of the present invention, chitosan is prepared by inoculating a filamentous fungi belonging to the family Mucoraceae into a culture medium for pre-cultivation containing yeast extract, malt extract, peptone, glucose and magnesium sulfate to perform pre-cultivation and then inoculating the pre-culture into a culture medium for main-cultivation having a composition essentially identical to that of the medium for pre-cultivation to perform the main-cultivation. Particularly preferred filamentous fungi belonging to the family Mucoraceae is Absidia coerulea. According to the method for preparing chitosan through the cultivation of microorganisms, chitosan exhibiting uniform quality can efficiently be produced and the method does not adversely affect the circumferential environment.

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Tokyo, Japan

Okmi Park