Dn Craft Corporation

Biobud Co., Ltd.

Ok Hwan Kim

Bum Joon Kim

Jae Hoon Hwang

Sung Yu Hong

Kwang Hoe Chung

Kyoung Jun Kim

Young-Doug Sohn

Wan Y Oh

Ji Hun Shin

The present invention relates to a batroxobin-encoding nucleotide sequence and/or a mutated &#x3b1;-factor secretion signal sequence, and a vector and a transformant using the same. The batroxobin-encoding nucleotide sequence of this invention exhibits an excellent expression efficiency in yeast, particularand the recombinant batroxobin is obtained at 4-13 fold higher yield than natural-occurring batroxobin-encoding sequences. The protein expression system which uses the batroxobin-encoding nucleotide sequence as well as mutated &#x3b1;-factor secretion signal peptide sequence of this invention obtains the recombinant batroxobin at about 20-fold higher yield than natural-occurring batroxobin-encoding sequences. In addition, the recombinant batroxobin prepared using the sequence of this invention has a significantly plausible activity and stability compared with natural-occurring batroxobin.

Mutated nucleotide sequences of batroxobin, mutated &#x3b1; factor secretion signal sequence and processes for preparing batroxobin using the same

A paper sheet decorative punching device is disclosed. In the punching device, a cap-shaped pressure member is movably received in an opening of a housing with two longitudinal guide pieces vertically extending from the lower edge of the pressure member. A punching blade unit, allowing the pressure member to be rotatable within a range, has two punching blade parts. The two blade parts have different punching patterns and different lengths. Both the pressure unit and the punching blade unit are biased upwardly by a compression coil spring, and so the pressure unit is normally projected from the top of the housing. Two edge guide members are symmetrically hinged to a base plate and are adjustable between a closed position for guiding a sheet's corner and an open position for guiding a sheet's side. A jig unit, seated on the base plate, consists of an upper holder member and a lower cutting member, with a paper sheet insert gap being formed between the two members. The holder member has two blade guide holes and two guide slots. The guide pieces of the pressure unit are selectively aligned with the guide slots in accordance with a rotated position of the pressure member. The cutting member has two cutting holes at positions corresponding to the two blade guide holes.

Paper sheet decorative punching device

Growing community of inventors

Seoul, South Korea

Ok Hwan Kim