The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization (aro) (volcani Center)

The State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organizatin (aro) (volcani Center)

State of Israel, Ministry of Agriculture & Rural Development, Agricultural Research Organization

State of Israel, Ministry of Agriculture and Rural Development Agricultural Research Organization, Volcani Center

Nurit Katzir

Arthur A Schaffer

Yosef Burger

Yaakov Tadmor

Shahar Cohen

Marina Petreikov

Efraim Lewinsohn

Ido Oz

Tamar Lavee

Fabian Baumkoler

Uzi Saar

Ayala Meir

Rachel Davidovich-rikanati

Adi Doron-faigenboim

Maxim Itkin

Vitaly Portnoy

Shmuel Shen

Elad Oren

Isolated mogroside and mogrol biosynthetic pathway enzyme polypeptides useful in mogroside biosynthesis are provided. Mogroside biosynthetic pathway enzymes of the invention include squalene epoxidase (SE), epoxy hydratase (EH), cytochrome p450 (Cyp), cucurbitadienol synthase (CDS) and udp-glucosyl-transferase (UGT), Also provided are methods of producing a mogroside using the isolated mogroside and mogrol biosynthetic enzyme polypeptides, the methods comprising contacting a mogrol and/or a glycosylated mogrol (mogroside) with at least one UDP glucose glucosyl transferase (UGT) enzyme polypeptide of the invention catalyzing glucosylation of the mogrol and/or the glucosylated mogrol to produce a mogroside with an additional glucosyl moietie(s), thereby producing the mogroside. Alternatively or additionally provided is a method of synthesizing a mogrol, the method comprising contacting a mogrol precursor substrate with one or more mogrol biosynthetic pathway enzyme polypeptides as described herein catalyzing mogrol synthesis from the mogrol precursor substrate, thereby synthesizing the mogrol.

Cells comprising mogroside pathway enzymes and uses thereof

Aplant or a part thereof, the plant bearing more than 12 fruit, the fruit being seedless. Methods of generating same and breeding same are also disclosed.

Melon plants with enhanced fruit yields

Provided are isolated polynucleotides comprising a nucleic acid sequence encoding a polypeptide, wherein the polypeptide modulates acidity of a plant. Also provided are nucleic acid constructs and plant cells comprising same and methods of using same for modulating acidity of a plant.

Isolated polynucleotides and methods and plants using same for regulating plant acidity

Growing community of inventors

Kiryat Tivon, Israel

Nurit Katzir