Kyowa Hakko Kogyo Co., Ltd.

Nozomu Kamada

Masato Ikeda

Tetsuo Nakano

Satoshi Koizumi

Yutaka Takano

Kazuyuki Okamoto

The present invention provides a protein having &#x3b1;1,2-fucosyltransferase activity, a DNA encoding the protein, a recombinant DNA comprising the DNA, a transformant comprising the recombinant DNA, a process for producing the protein having &#x3b1;1,2-fucosyltransferase activity using the transformant, and a process for producing a fucose-containing complex carbohydrate using the transformant.

&#x3b1;1,2-fucosyltransferase and DNA encoding the same

Attempts are made to provide a novel transaldolase gene; a polypeptide encoded by this gene; a recombinant DNA obtained by integrating this gene; a microorganism carrying this recombinant DNA; and a process for producing an aromatic amino acid, an aromatic vitamin, L-histidine, riboflavin, a nucleic acid, a nucleic acid-associated substance, a novel saccharide, etc. by using the above microorganism. As the results of extensive studies, a novel transaldolase gene is isolated from chromosomal DNA of a microorganism belonging to the genusas a DNA fragment complementary to the requirement for shikimic acid of a transketolase defective variant obtained as a variant with the requirement for shikimic acid belonging to the genus. Further, a recombinant DNA containing this gene is constructed and transferred into a host microorganism, thereby achieving the objects as described above.

Transaldolase gene

An industrially more advantageous method for the production of metabolites biologically synthesized via phosphoribosyl pyrophosphate (PRPP) is provided, making use of metabolically modified strains in which transketolase activity is deficient or reduced in comparison with the parent strain.

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Hofu, Japan

Nozomu Kamada