Emulate, Inc.

Harvard College

Cedars-sinai Medical Center

Janssen Biotech, Inc.

Wen, Norman

Novak, Richard

Fernandez-alcon, Jose

Norman Wen

Daniel Levner

Christopher David Hinojosa

Geraldine A Hamilton

Antonio Varone

Jacob Fraser

S Jordan Kerns

Justin Nguyen

Guy Robert Thompson, Ii

Catherine Karalis

Lewis Rowe

Clive N Svendsen

Dhruv Sareen

Riccardo Barrile

David Conegliano

Carol Lucchesi

Jefferson Puerta

Donald Elliot Ingber

Josiah Daniel Sliz

Richard Novak

Hyoungshin Park

Monicah Otieno

Andries Van Der Meer

Gad Vatine

Robert N Barrett

Carolina Lucchesi

Magdalena Kasendra

Samuel Sances

Stephen R Targan

Michael Workman

Uthra Rajamani

Lori McPartlin

Youngjae Choe

Lina Williamson

Joshua Gomes

Chris Hinojosa

Matthew Daniel Solomon

Jacob Freake

Doug Sabin

Lian Leng

Petrus Wilhelmus Martinus Van Ruijven

Christian Alexander Potzner

Patrick Sean Tuohy

Grace Ahn

Brian Zuckerman

Tanvi Shroff

Victor Antontsev

Jonathan Rubins

Hyoung Shin Park

Jose Fernandez-Alcon

Remi Villenave

Dan Gazit

Gadi Pelled

Guy Thompson

Zulma Gazit

Georg Bauer

Dmitriy Sheyn

Alfred Paris

Karel Domansky

Kambez Hajipouran Benam

Thomas Umundum

Ville Kujala

David James Coon

Tiama Hamkins-Indik

SJordan Kerns

Samuel Sanees

Miles Ingram

Daniel E Shea

Ii Thompson Guy Robert

Ii Guy Thompson

James Coon

Guy Robert Thompson Ii

Petrus Martinus Van Ruijven

Doug Doug

Justin K Nguyen

Magdelena Kasendra

Jordan Kerns

Guy Robert Thompson

The invention relates to culturing brain endothelial cells, and optionally astrocytes and neurons in a fluidic device under conditions whereby the cells mimic the structure and function of the blood brain barrier. Culture of such cells in a microfluidic device, whether alone or in combination with other cells, drives maturation and/or differentiation further than existing systems.

Microfluidic model of the blood brain barrier

Organs-on-chips are microfluidic devices for culturing living cells in micrometer sized chambers in order to model physiological functions of tissues and organs. Engineered patterning and continuous fluid flow in these devices has allowed culturing of intestinal cells bearing physiologically relevant features and sustained exposure to bacteria while maintaining cellular viability, thereby allowing study of inflammatory bowl diseases. However, existing intestinal cells do not possess all physiologically relevant subtypes, do not possess the repertoire of genetic variations, or allow for study of other important cellular actors such as immune cells. Use of iPSC-derived epithelium, including IBD patient-specific cells, allows for superior disease modeling by capturing the multi-faceted nature of the disease.

Systems and methods for growth of intestinal cells in microfluidic devices

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Open-top microfluidic devices and methods for simulating a function of a tissue

The present invention is related to the field of microfluidics and compound distribution within microfluidic devices and their associated systems. In one embodiment, present invention aims to solve the problem of molecule and compound absorbency into the materials making up laboratory equipment, microfluidic devices and their related infrastructure, without unduly restricting gas transport within microfluidic devices.

Compound distribution in microfluidic devices

Development of spinal cord on a microfluidic chip

An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Organomimetic devices and methods of use and manufacturing thereof

Microfluidic 'organ-on-a-chip' devices have been developed with the aim to replicate human tissues in vitro. However, there is no option to quantitatively monitor biological processes that take place within the chip, over time. Destructive methods in order to analyze, tissue formation, gene expression, protein secretion etc. require the harvest of the “tissue” at a certain time point. Described herein are methods and compositions for non-destructive molecular imaging methods and systems in order to quantitatively monitor specific biological processes, over time, within the chip, without the need to harvest.

Method of osteogenic differentiation in microfluidic tissue culture systems

The invention generally relates to a microfluidic platforms or 'chips' for testing and understanding cancer, and, more specifically, for understanding the factors that contribute to cancer invading tissues and causing metastases. Tumor cells are grown on microfluidic devices with other non-cancerous tissues under conditions that simulate tumor invasion. The interaction with immune cells can be tested to inhibit this activity by linking a cancer chip to a lymph chip.

Devices, systems and methods for inhibiting invasion and metasases of cancer

A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall surfaces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.

Open-top microfluidic device with structural anchors

A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.

Method for micromolding a polymeric membrane having a pore array

Growing community of inventors

West Roxbury, MA, United States of America

Norman Wen