Charles River Laboratories, Inc.

Reametrix Inc.

Associates of Cape Cod, Incorporated

Gelman Sciences, Inc.

Marine Biological Laboratory

Norman R Wainwright

Eric Stimpson

Bala S Manian

Thomas Preidel

Dana M Nutter

Brian J Kolonia

Robert K Kolonia

Foster T Jordan

Al Fuchs

Thomas J Novitsky

Bruce J Tomson

Bruce Joni Tomson

James F Cooper

Hui-Ti Chiang

Steven H Boyd

Masakazu Tsuchiya

Frank C Rumore

Charles Balas, Jr

John Dubczak

The invention relates generally to hybrid amebocyte lysate compositions (including both native and recombinant components) and their use in detecting and/or quantifying endotoxin in a sample.

Recombinant amebocyte lysate and uses thereof

The invention relates to a porous planar cell capture system for use in determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using such a cell capture system. The cell capture system contains a fluid permeable, planar membrane adopted to retain cells thereon, a fluid permeable support member that supports the membrane, and an optional register associated with the membrane.

Porous planar cell capture system and method of use

The invention relates to a filtration system for use in a method of determining the presence and/or amount of cells, for example, viable cells, in a liquid sample, and to methods of using and manufacturing such a filtration system. The filtration system includes a cup with an upper portion and a ring portion, where the ring portion is separably coupled to the upper portion.

Filtration system and use thereof

Porous planar cell capture system

The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay.

Compositions for the detection of microbial contaminants

The invention relates to a method for determining the presence and/or amount of viable cells in a liquid sample. The method includes (a) labeling with a fluorescent label viable cells remaining on a porous membrane after passing the liquid sample therethrough, (b) scanning a portion of the porous membrane by rotating the porous membrane relative to a detection system, and (c) determining the presence and/or quantity of viable cells captured by the membrane.

Growing community of inventors

Johns Island, SC, United States of America

Norman R Wainwright