Garst Seed Company

Zenco (no. 4) Limited

Norman P Cloud

John A Greaves

Raymond Russotti

Martin A Stoecker

Daren Kenneth Coonrod

Raymond R Russotti

An apparatus for the preparation of multiple pollen samples for cryogenic preservation comprising a plurality of chambers for the storage of pollen samples. A vacuum pump reduces the pressure within the interior of the chambers through a vacuum manifold that operatively connects with the pollen samples in the interior of the chambers. An air source vents the interior of the chambers to atmospheric pressure through an air manifold operatively connected with the pollen samples in the interior of the chambers. A vacuum controller controls the pressure within the interior of the chambers through operative connections to the vacuum manifold, the air manifold, the air source, and the vacuum pump. The vacuum controller pulses the pressure with the interior of the chambers between a first and a second reduced pressure level. A sensor operatively connected to the chambers senses the moisture content of the pollen samples within the interior of the chambers at the reduced pressure levels. After the pollen samples reach a predetermined moisture content, the pollen samples can be cyrogenically preserved in a pollen bank for later plant-breeding use.

Apparatus for the preparation of multiple pollen samples for cryogenic

The present invention relates to a method and instrument for the preparation of pollen and the development of a pollen bank for breeding purposes. More specifically, the invention relates to a method and instrument that permits pollen to be cryogenically stored in a viable state. Specifically, the present invention relates to a method of employing a heat or water or pressure related measurement to determine the readiness of the pollen for cryogenic storage.

Method and instrument for the preparation of pollen for cryogenic storage

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Pleasanton, TX, United States of America

Norman P Cloud