Gen-probe, Inc.

Qualigen, Inc.

Aegea Biotechnologies, Inc.

Kel-lite Industries, Inc.

Other

Streamlight, Inc.

Michael Becker

Norman C. Nelson

Norman C Nelson

Steven T Brentano

Lyle J Arnold

Richard S Lee

Jason Taylor

Matthew J Scott

Scott S Breidenthal

Michael McClellan Becker

Lyle J Arnold, Jr

Jijumon Chelliserry

Dmitry Lyakhov

James D Carlson

Sara H Fan

Lizhong Dai

Jason A Taylor

Daniel L Kacian

Ian Weeks

James Stuart Woodhead

Steven Scott Phelps

Margarita Batranina-Kaminsky

Azzouz Ben Cheikh

James John Hogan

Kristin W Livezey

Gary P Schroth

James Russell

Kenneth A Browne

Margarita B Kaminsky

Robert Bezverkov

Gianluca Roma

Mark E Filipowsky

Jr Arnold Lyle J

David Opalsky

Sherrol Hoffa McDonough

Mark A Reynolds

Haitao Li

Daniel Kolk

George T Walker

Wai-Chung Lam

Michael Poirier

Robert E Heinz

Jorge Velarde

Astrid R W Schroder

Alexander A Waldrop, Iii

Matthew C Friedenberg

Anne-Laure Shapiro

Scott Breidenthal

Michael S Poirier

Steven S Phelps

Matthew Friedenberg

Richard Lee

Matthew Scott

Sherrol H Mcdonough

Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed.

Multiphase nucleic acid amplification

Methods for selecting tag-oligonucleotide sequences for use in an in vitro nucleic acid assay. The selected tag sequences are useful for nucleic acid assay wherein interference between the nucleic acid sequences is the assay is to be controlled. Selected tag sequences are incorporated into nucleic acid assay to improve the performance of and/or minimize any interference between nucleic acids in the assay compared to untagged assays.

Methods and compositions for the selection and optimization of oligonucleotide tag sequences

Disclosed are methods for amplifying a nucleic acid target region using an amplification oligomer comprising a target-binding segment and a heterologous displacer tag situated 5' to the target-binding segment. Initiation of an amplification reaction from the tagged amplification oligomer produces an amplicon comprising the displacer tag, such that once the complement of the displacer tag has been incorporated into a second amplicon, a displacer oligonucleotide having a sequence substantially corresponding to the displacer tag sequence is used to participate in subsequent rounds of amplification for displacement of an extension product primed from a site within the second amplicon 5&#x2032; to the displacer priming site. Also disclosed are related kits and reaction mixtures comprising the displacer-tagged amplification oligomer and corresponding displacer oligonucleotide.

Methods for amplifying nucleic acid using tag-mediated displacement

The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications.

Closed nucleic acid structures

A receptacle comprises opposed members, a plurality of chambers having perimeter walls defined by seals formed between the opposed members and portals interconnecting the chambers, and a rigid frame supporting the opposed members at their peripheral edges. The frame comprises a front frame portion and a rear frame portion, and the peripheral edges of the opposed members are retained between the front and rear frame portions. An inlet port extends between the front and rear frame portions and is in fluid communication with one of the chambers.

System and method of using multi-chambered receptacles

A method of processing a sample in a receptacle comprising a plurality of chambers. Each of the chambers is connected to at least one other chamber by a portal and at least a first one of the chambers is formed of a flexible material. The method includes the steps of causing gas bubbles contained in the first chamber to accumulate in a portion of the first chamber, applying a compressive external force to the first chamber to cause some or all of the liquid contents of the first chamber to flow into an interconnected second chamber through a portal connecting the first and second chambers; and preventing the gas bubbles accumulated in a portion of the first chamber from flowing through the portal into the second chamber.

A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle.

Multi-chambered receptacles

Compositions, reaction mixtures, and methods for performing an amplification reaction, including multiplex amplification reaction, wherein the method comprises using one or more amplification oligomer complexes comprising linked first and second amplification oligomer members. In one aspect, the amplification oligomer complex is hybridized to a target nucleic acid, the target nucleic acid with hybridized amplification oligomer complex is then captured, and other components are washed away. Target sequences of the target nucleic acids are pre-amplified to generate a first amplification product. The first amplification product is amplified in one or more secondary amplification reactions to generate second amplification products.

Methods and compositions for nucleic acid amplification

The present invention provides methods of amplifying a target nucleic acid utilizing a circularized template. Circularization may be achieved utilizing a bridging oligonucleotide or an inverter primer. The bridging oligonucleotide or inverted primer is extended forming a concatemeric amplicon that can then be used as a template to provide exponential amplification of the target nucleic acid.

Methods for amplification of nucleic acids utilizing a circularized template prepared from a target nucleic acid

Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample.

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San Diego, CA, United States of America

Norman C Nelson