The United States of America As Represented by the Secretary of

Delta and Pine Land Company

Cotton, Inc.

Southplains Biotechnologies, Inc.

Norma Lee Trolinder

Don Lee Keim

Melvin John Oliver

Jerry Edwin Quisenberry

Linda Kay Koonce

Jane K Dever

Jane Gay Dever

This invention relates to methods for transforming cotton using isolated shoot apical tips of seedlings. The apical shoot tips of cotton seedlings are isolated and chilled at a low temperature to slow the metabolic activity of the isolated cells, and then inoculated with a transforming agent carrying a desired gene. The invention also provides a method of regenerating transformed cotton plants with a desired trait from transformed apical shoot tips.

Methods for producing transgenic cotton plants using chilled apical shoot tips

A method for in vitro regeneration of fertile Gossypium plants is provided in which cells from the transition region tissue of seedlings is excised and cultured. The transition region tissue of cotton seedlings extends from the uppermost portion of the root and into the hypocotyl region. Transformed cells are regenerated into homogeneously transformed plants by means of somatic embryogenesis on hormone-free medium. A method for production of transgenic Gossypium plants capable of transmitting a foreign gene to progeny is also described in which cells derived from the transition region tissue of seedlings are targeted for transformation. The method increases the number of different cotton genotypes that can be used to make stably transformed plants capable of transmitting the foreign gene to progeny.

Transformation and regeneration of fertile cotton plants

A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also a method for making a genetically modified hybrid plant by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.

Control of plant gene expression

Growing community of inventors

Quanah, TX, United States of America

Norma Lee Trolinder