Chisso Corporation

Jnc Corporation

National Institute of Advanced Industrial Science and Technology

Agency of Industrial Science and Technology

Japan Chemical Innovation Institute

Toyohashi University of Technology

Kobe University

Ortho-Clinical Diagnostics Kabushiki Kaisha

Noriyuki Ohnishi

Kazutoshi Miyazawa

Etsuo Nakagawa

Yasuyuki Goto

Kazunori Kataoka

Katsuhiko Ueno

Shuichi Matsui

Shinichi Sawada

Tomoyuki Kondo

Hirotaka Furukawa

Kageaki Matsui

Yasuhiro Haseba

Masaru Eguchi

Kazumi Aoshima

Hiroyuki Takeuchi

Atsuko Fujita

Satoru Sugita

Hirokazu Nagaoka

Saburo Tanaka

Xiaomao Xie

Shinji Katsura

Hideyuki Hata

Fusayuki Takeshita

Takashi Kato

Takashi Katoh

Akihiko Kondo

Norihisa Hachiya

Yasuyuki Koizumi

Yasusuke Hisatsune

Yuki Takahashi

Takako Nogami

Hideo Nishizawa

Katsuhiko Kitsugi

Mikiko Yoshida

The present invention provides a method for efficiently separating cesium ions in a short time from an aqueous solution with the number of human working steps being reduced as much as possible and recovering the cesium ions, and an apparatus therefor. Cesium ions in an aqueous solution are removed by preparing a cesium ion-containing magnetic particle in a cesium-containing aqueous solution and filtering or magnetically separating the magnetic particle.

Method for removing cesium ions from water

The present invention provides a method for measuring oocyst of protozoa, such as, in an environment sample with high sensitivity at low cost within a short period of time; and a detecting reagent for use therein. Magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, binding factors for specific recognition of oocyst are added to an analyte containing a protozoan oocyst to form oocyst/binding factor/magnetic fine particle complexes by using a binding reaction to the oocyst, the formed complexes are recovered by a magnetic separation, and the protozoan oocysts contained in the complexes are assayed. Further, there is provided, for conducting the above method, a reagent for detecting protozoan oocysts comprising magnetic fine particles of 5 to 500 nm particle diameter having, immobilized thereto, antibodies against oocysts or binding factors for recognizing the antibodies.

Method for measuring protozoan oocyst and detecting reagent

Provided are a detection method and a quantification method for a detection target, which can detect and quantify the detection target rapidly, inexpensively, simply, and with high accuracy in a variety of environments. A method for detecting the detection target in a specimen includes processes in which there are mixed a first binding substance which binds a first material containing a stimuli-responsive polymer and a first affinity substance which has affinity for the detection target, a second binding substance which binds a hydrophilic second material and a second affinity substance which has affinity for the detection target, and a specimen; the resulting mixture is placed under conditions where the stimuli-responsive polymer agglutinates and the mixture is developed into a developing carrier, or the resulting mixture is developed into a developing carrier and the mixture is placed under conditions where the stimuli-responsive polymer agglutinates; the signal produced by the presence of the first binding substance or the second binding substance in the developing carrier is verified; and the presence of the detection target in the specimen is determined when the signal is different from that when the detection target is absent. The first affinity substance and the second affinity substance can bind to different positions of the detection target.

Detection method and quantification method for target substance

Disclosed are a detection/quantification kit and a detection/quantification method for detecting/quantifying an analyte rapidly at a low cost and in a simple manner. Specifically, disclosed is a kit for detecting an analyte in a sample, comprising a first conjugate and a second conjugate, wherein the first conjugate comprises a first substance comprising a stimuli-responsive polymer and a first affinity substance having affinity for the analyte and bound to the first substance, and the second conjugate comprises a second substance carrying an electrical charge and a second affinity substance having affinity for the analyte and bound to the second substance. The first and second affinity substances can bind to different sites on the analyte simultaneously.

Kit for detection/quantification of analyte, and method for detection/quantification of analyte

A sensing method for biopolymers by detecting the magnetic signal generated from a labeled biopolymer under AC magnetic field with thermo-responsive magnetic nano particles as label having a critical solution temperature across which the particles have the ability to aggregate or disperse with cooling or heating.

Sensing method for biopolymers and sensing device therefor

The present invention provides a highly sensitive sensing device for biopolymers which detects the magnetic signal generated from a labeled biopolymer under AC magnetic field using thermo-responsive magnetic nano particles as a probe.

The present invention relates to magnetic fine particles having a lower critical solution temperature to which at least one substance selected from biotin and avidin is immobilized, and a method of converting a substance, a method of separating or concentrating a microorganism, a method of modifying a denatured protein, a method of detecting a nucleic acid, a separating agent, and a method of separating a biological substance using the same.

Magnetic fine particles having lower critical solution temperature

It is intended to provide a method capable of simply aggregating magnetic particles having a surface modified with a thermoresponsive polymer at a given temperature without heating or cooling an aqueous solution containing the magnetic particles, and a separation method and a detection method of a substance to be detected in a sample using the method. The method of separating a substance to be detected from a sample includes the steps of: mixing an adsorbent and the sample in an aqueous solution to adsorb the substance to be detected on the adsorbent, aggregating the adsorbent by changing a salt concentration in the aqueous solution; and collecting the adsorbent from the aqueous solution by a magnetic force, wherein said adsorbent comprises a magnetic particle of an average particle size of 50 to 1000 nm, a surface of which is modified with a thermoresponsive polymer and is immobilized with a substance having an affinity for the substance to be detected.

Aggregation and dispersion methods of magnetic particles, separation and detection methods using the same and detection kit

It is intended to provide an aqueous solution of a thermoresponsive polymer, which shows an upper critical solution temperature (UCST) and is capable of maintaining a dissolved state, even if it is stored for a long period of time at a temperature at which a ligand such as an antibody or an antigen is not inactivated. A UCST lowering agent is incorporated in a predetermined amount in an aqueous solution of a thermoresponsive polymer which shows a UCST in a state of containing water, thereby lowering the UCST of the aqueous solution to less than the storage temperature, and the resulting aqueous solution is stored.

Method of storing thermoresponsive polymer in state of aqueous solution

It is intended to provide an aqueous solution of a thermoresponsive polymer and an aqueous dispersion of a thermoresponsive particle, which show an upper critical solution temperature (UCST) and are capable of maintaining a dissolved state and a dispersed state, respectively, even if they are stored for a long period of time at a temperature at which a ligand such as an antibody or an antigen is not inactivated. A UCST lowering agent is incorporated in a predetermined amount in an aqueous solution of a thermoresponsive polymer which shows a UCST in a state of containing water or an aqueous dispersion of a thermoresponsive particle containing the polymer, thereby lowering the UCST of the aqueous solution or aqueous dispersion to less than the storage temperature, and the resulting aqueous solution or aqueous dispersion is stored.

Aqueous solution of polymer having upper critical solution temperature, aqueous dispersion of particle modified with the polymer and method of storing the same

A material which expresses its critical solution temperature even in an aqueous solution containing a buffer or salt, and at the same time, shows small variation width of the critical solution temperature even when concentration of salt or the like contained in water, a buffer liquid or the like is different, interacts with a substance such as a component in the living body and can therefore be used efficiently in the isolation of substances, is provided. A monomer represented by a formula (1) and a thermoresponsive polymer which contains said monomer as a polymerization component.

Thermoresponsive polymer and production method thereof

A stimuli-responsive polymer derivative utilizing keto-enol tautomerization. Also disclosed are a simple process for producing an N-acyl(meth)acrylamide derivative which can be used as a monomer for the stimuli-responsive polymer, a process for the production of an intermediate thereof, and an intermediate thus produced.

Stimuli-responsive polymer utilizing keto-enol tautomerization and stimuli-responsive separating material and chemical-releasing capsule comprising the same

The present invention relates to magnetic fine particles having immobilized thereto a polymer having an upper critical solution temperature, and a process for producing the same, a method of separating or concentrating a microorganism, a method of purifying, detecting, or concentrating a nucleic acid, a separating agent, a method of separating a biological substance, and a method of converting a substance.

Magnetic fine particles and process for producing the same

The invention relates to a stimulus-responding polymer obtained by polymerizing at least a monomer represented by a general formula (1) and a monomer represented by a general formula (2), and a separation method or concentration method of microorganisms, a purification method, detection method or concentration method of nucleic acids, a separating agent, a separation method of biomaterials and a conversion method of materials, which use this polymer, (in the formula, Rrepresents hydrogen atom or methyl group, and Rrepresents single bond or a straight or branched alkylene group having from 1 to 5 carbon atoms) (in the formula, Rrepresents hydrogen atom or methyl group, and Rrepresents hydrogen atom, a straight, branched or cyclic alkyl group, alkoxyl group or alkylamino group having from 1 to 10 carbon atoms, an aryl group or a heterocyclic group).

Growing community of inventors

Chiba, Japan

Noriyuki Ohnishi