Tosoh Corporation

Noriyoshi Masuda

Kiyoshi Yasukawa

Takahiko Ishiguro

Ryuichi Horie

Toshiki Taya

Juichi Saito

Takahiro Maruyama

Shigeo Tsuchiya

Takao Matsuba

Yuichi Isawa

A detection reagent for detecting thermostable direct hemolysin-related hemolysin (TRH) gene in an amplification process which specifically amplifies TRH1 and TRH2 RNA, which reagent comprises a first primer having a sequence complementary to a specific sequence of an RNA derived from the TRH gene, and a second primer having a sequence homologous to said specific sequence.

[object Object]

The present invention provides a combination of oligonucleotides preferable for composing a rapid and specific gene testing reagent for Shiga toxin family gene type 2 (stx) of entero-hemorrhagic(EHEC). More specifically, the present invention provides a method for detecting stx2 RNA of EHEC by specifically amplifying only stx2 RNA using a primer having a sequence homologous or complementary to a base sequence specific for stx2 gene of EHEC and located at sites free of alterations between genotypes, and an oligonucleotide that binds to a specific site of stx2 RNA.

Nucleic acid sequences, oligonucleotides and a method for detection of SRSV, in particular, a virus which belongs to Genotype II (GII), in clinical examinations, public health examinations, food evaluations and food poisoning examinations are provided.

Oligonucleotides and method for characterizing and detecting Genogroup II type small round structured virus

An oligonucleotide, capable of specific cleavage and amplification of a pab gene which codes for pab, an antigenic protein of, or an RNA derived therefrom, as well as being useful in high sensitive detection and identification thereof, is provided. Further, a combination of oligonucleotides, useful in specific amplification, high sensitive detection and identification of an RNA derived frompab gene as well as a process for detectingby using said combination, are provided.

Oligonucleotides for detecting tubercle bacillus and method therefor

A method of detecting a specific acid-fast bacterium, wherein primers having nucleotide sequences homologous or complementary to the specific regions in the rRNAs of the specific acid-fast bacterium are used to specifically amplify only the rRNAs of the specific acid-fast bacterium.

Growing community of inventors

Tokyo, Japan

Noriyoshi Masuda