E.i. Dupont De Nemours and Company

Japan Tobacco Inc.

Pioneer Hi-Bred International, Inc.

Riken Corporation

Pioneer Hi-Bred Int'l Inc.

Norio Kato

Marc C Albertsen

Hajime Sakai

Yoshimitsu Takakura

Stanley Luck

Scott V Tingey

Yukoh Hiei

Toshihiko Komari

Ichiro Oka

Satoru Usami

Toshiyuki Komori

Teruyuki Imayama

Masakazu Kashihara

Takashi Okamoto

Takatoshi Kiba

Erika Toda

The present invention relates to a method for introducing a substance into a plant. The method of the present invention comprises the steps of: obtaining an enzymatically treated and isolated fertilized egg cell by (1-i) isolating a fertilized egg cell from a plant tissue containing a fertilized egg cell, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, (1-ii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the fertilized egg cell that has been enzymatically treated, (1-iii) treating a plant tissue containing a fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and simultaneously isolating the fertilized egg cell that has been enzymatically treated, (1-iv) isolating an egg cell and a sperm cell from a plant to produce a fertilized egg by fusing the cells, and then treating the fertilized egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, or (1-v) treating a plant tissue containing an egg cell with an enzyme solution containing a plant tissue-degrading enzyme under a low-titer condition, and then isolating the egg cell that has been enzymatically treated, and further fusing the egg cell with an isolated sperm cell; and (2) introducing a substance selected from the group consisting of nucleic acids, proteins, and peptides into the resultant enzymatically treated and isolated fertilized egg cell.

Method for introducing substance into plant

Isolated polynucleotides and polypeptides and recombinant DNA constructs useful for conferring drought tolerance, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a DTP21 polypeptide.

Drought tolerant plants and related constructs and methods involving genes encoding DTP21 polypeptides

Growing community of inventors

Shizuoka, Japan

Norio Kato